Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 526793..527429 Replicon chromosome
Accession NZ_CP050705
Organism Bacillus atrophaeus strain PENSV20

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HCU65_RS02620 Protein ID WP_003156187.1
Coordinates 527079..527429 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A837XXL9
Locus tag HCU65_RS02615 Protein ID WP_003328141.1
Coordinates 526793..527074 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCU65_RS02595 (523089) 523089..523688 - 600 WP_106034462.1 rhomboid family intramembrane serine protease -
HCU65_RS02600 (523788) 523788..524153 + 366 WP_106034463.1 holo-ACP synthase -
HCU65_RS02605 (524321) 524321..525337 + 1017 WP_106034464.1 outer membrane lipoprotein carrier protein LolA -
HCU65_RS02610 (525505) 525505..526674 + 1170 WP_003328140.1 alanine racemase -
HCU65_RS02615 (526793) 526793..527074 + 282 WP_003328141.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HCU65_RS02620 (527079) 527079..527429 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HCU65_RS02625 (527545) 527545..528363 + 819 WP_003328142.1 RsbT co-antagonist protein RsbRA -
HCU65_RS02630 (528368) 528368..528733 + 366 WP_010789626.1 RsbT antagonist protein RsbS -
HCU65_RS02635 (528736) 528736..529137 + 402 WP_003328144.1 serine/threonine-protein kinase RsbT -
HCU65_RS02640 (529149) 529149..530156 + 1008 WP_063638786.1 PP2C family protein-serine/threonine phosphatase -
HCU65_RS02645 (530216) 530216..530545 + 330 WP_003328146.1 anti-sigma factor antagonist RsbV -
HCU65_RS02650 (530542) 530542..531024 + 483 WP_003328147.1 anti-sigma B factor RsbW -
HCU65_RS02655 (530990) 530990..531778 + 789 WP_061572301.1 RNA polymerase sigma factor SigB -
HCU65_RS02660 (531778) 531778..532377 + 600 WP_219947088.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T153131 WP_003156187.1 NZ_CP050705:527079-527429 [Bacillus atrophaeus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T153131 NZ_CP066759:3468297-3468584 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10451.83 Da        Isoelectric Point: 5.1903

>AT153131 WP_003328141.1 NZ_CP050705:526793-527074 [Bacillus atrophaeus]
MSESSATTEMMISLPEALVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT153131 NZ_CP066759:3468106-3468297 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB A0A837XXL9

References