Detailed information of TA system TA153123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 879720..880737
• Replicon: chromosome
• Accession: NZ_CP050695
• Organism: Aquisalimonas sp. 2447

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HC341_RS04025
• Protein ID: WP_167784109.1
• Coordinates: 880222..880737 (+)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: HC341_RS04020
• Protein ID: WP_167781412.1
• Coordinates: 879720..880172 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC341_RS04000	874860..876263	+	1404	WP_174813712.1	hypothetical protein	-
HC341_RS04005	876611..877240	+	630	WP_167781409.1	YceI family protein	-
HC341_RS04010	877334..878707	+	1374	WP_167781410.1	MFS transporter	-
HC341_RS04015	878926..879561	-	636	WP_167781411.1	tyrosine-type recombinase/integrase	-
HC341_RS04020	879720..880172	+	453	WP_167781412.1	helix-turn-helix domain-containing protein	Antitoxin
HC341_RS04025	880222..880737	+	516	WP_167784109.1	PIN domain-containing protein	Toxin
HC341_RS04030	880939..881295	-	357	WP_167781413.1	hypothetical protein	-
HC341_RS04035	881376..881864	-	489	WP_167781414.1	NADH-quinone oxidoreductase subunit NuoI	-
HC341_RS04040	881911..882387	-	477	WP_167781220.1	NADH-quinone oxidoreductase subunit B	-
HC341_RS04045	882433..885171	-	2739	WP_167781415.1	aconitate hydratase AcnA	-
HC341_RS04050	885228..885566	-	339	WP_167781416.1	(2Fe-2S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	819967..940831	120864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-94)

Antitoxin

• Helix-turn-helix domain, group 17 (80-128)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 18858.52 Da
• Isoelectric Point: 5.2584

>T153123 WP_167784109.1 NZ_CP050695:880222-880737 [Aquisalimonas sp. 2447]
MRDFLIRLATAGLFAAKWTDRIHDEWTRSLLATRPDLADALKRTRSLMDQAVPDCLVEGYEPLIEGLSLPDPDDRHVLAA
AIRAGAQTIVTINLRDFPAPSLAPYGIEAVHPDSFVEQQLDLHEGAVVTTAKRHRAALRNPPKSVDAYLETLAAQGLVVT
ADRLREFADVL

Nucleotide

• Length: 516 bp

>T153123 NZ_CP066759:890632-891039 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16549.60 Da
• Isoelectric Point: 4.8149

>AT153123 WP_167781412.1 NZ_CP050695:879720-880172 [Aquisalimonas sp. 2447]
MQTPSGDNQLPDHEAAALARASATELSRLLAQQPDSDRARVQLDGTDLVLPRQALALLRDLLGEMAQGNAVTIVPTHAEL
TTQEAADILNVSRPHLVKLLERGELPFSKVGTHRRIRYQDLLAYKAERDRTSQEALDALTEQAQDLDMGY

Nucleotide

• Length: 453 bp

>AT153123 NZ_CP066759:890385-890651 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGAGC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA