Detailed information of TA system TA153117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2646478..2647007
• Replicon: chromosome
• Accession: NZ_CP050691
• Organism: Staphylococcus aureus strain PMB196-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HCU71_RS12995
• Protein ID: WP_000621175.1
• Coordinates: 2646478..2646840 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HCU71_RS13000
• Protein ID: WP_000948331.1
• Coordinates: 2646837..2647007 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCU71_RS12975	2643457..2644227	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
HCU71_RS12980	2644202..2644681	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
HCU71_RS12985	2644683..2645009	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HCU71_RS12990	2645128..2646129	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HCU71_RS12995	2646478..2646840	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HCU71_RS13000	2646837..2647007	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HCU71_RS13005	2647092..2648240	-	1149	WP_001281154.1	alanine racemase	-
HCU71_RS13010	2648306..2648665	-	360	WP_000581197.1	holo-ACP synthase	-
HCU71_RS13015	2648669..2649160	-	492	WP_001205912.1	PH domain-containing protein	-
HCU71_RS13020	2649147..2650731	-	1585	Protein_2514	PH domain-containing protein	-
HCU71_RS13025	2650724..2651203	-	480	WP_001287087.1	hypothetical protein	-
HCU71_RS13030	2651411..2651971	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T153117 WP_000621175.1 NZ_CP050691:c2646840-2646478 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T153117 NZ_CP066759:309523-309798 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT153117 WP_000948331.1 NZ_CP050691:c2647007-2646837 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT153117 NZ_CP066759:309795-310154 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1