Detailed information of TA system TA153103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1852482..1853011
• Replicon: chromosome
• Accession: NZ_CP050690
• Organism: Staphylococcus aureus strain PMB179-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HCU70_RS09045
• Protein ID: WP_000621175.1
• Coordinates: 1852649..1853011 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HCU70_RS09040
• Protein ID: WP_000948331.1
• Coordinates: 1852482..1852652 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCU70_RS09010	1847518..1848078	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
HCU70_RS09015	1848287..1848766	+	480	WP_001287079.1	hypothetical protein	-
HCU70_RS09020	1848759..1850342	+	1584	WP_001294620.1	PH domain-containing protein	-
HCU70_RS09025	1850329..1850820	+	492	WP_001205912.1	PH domain-containing protein	-
HCU70_RS09030	1850824..1851183	+	360	WP_000581202.1	holo-ACP synthase	-
HCU70_RS09035	1851249..1852397	+	1149	WP_000130547.1	alanine racemase	-
HCU70_RS09040	1852482..1852652	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HCU70_RS09045	1852649..1853011	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HCU70_RS09050	1853360..1854361	+	1002	WP_138390383.1	PP2C family protein-serine/threonine phosphatase	-
HCU70_RS09055	1854480..1854806	+	327	WP_001052491.1	anti-sigma factor antagonist	-
HCU70_RS09060	1854808..1855287	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
HCU70_RS09065	1855262..1856032	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T153103 WP_000621175.1 NZ_CP050690:1852649-1853011 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T153103 NZ_CP066756:4118942-4119160 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT153103 WP_000948331.1 NZ_CP050690:1852482-1852652 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT153103 NZ_CP066756:4118542-4118916 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1