Detailed information of TA system TA153092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 135031..135767
• Replicon: plasmid pNDM-IMP-2
• Accession: NZ_CP050682
• Organism: Klebsiella variicola strain SHET-01

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HCO75_RS29070
• Protein ID: WP_274757501.1
• Coordinates: 135285..135767 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HCO75_RS29065
• Protein ID: WP_003026799.1
• Coordinates: 135031..135297 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCO75_RS29040 (HCO75_29330)	130335..131786	-	1452	WP_046622880.1	L-tyrosine/L-tryptophan isonitrile synthase family protein	-
HCO75_RS29045 (HCO75_29335)	132028..133059	+	1032	WP_274757509.1	GlxA family transcriptional regulator	-
HCO75_RS29050 (HCO75_29340)	133110..133272	+	163	Protein_129	type I toxin-antitoxin system Hok family toxin	-
HCO75_RS29055 (HCO75_29345)	133441..133728	-	288	WP_046622894.1	chorismate mutase	-
HCO75_RS29060 (HCO75_29350)	133877..134710	-	834	WP_274757500.1	hypothetical protein	-
HCO75_RS29065 (HCO75_29355)	135031..135297	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HCO75_RS29070 (HCO75_29360)	135285..135767	+	483	WP_274757501.1	GNAT family N-acetyltransferase	Toxin
HCO75_RS29075 (HCO75_29365)	135983..137383	+	1401	WP_131024503.1	ISNCY family transposase	-
HCO75_RS29080 (HCO75_29370)	137571..137990	+	420	WP_012540023.1	ASCH domain-containing protein	-
HCO75_RS29085 (HCO75_29375)	138572..139573	+	1002	WP_040107896.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..144371	144371
	-	flank	IS/Tn	-	-	135983..137383	1400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17375.04 Da
• Isoelectric Point: 9.5825

>T153092 WP_274757501.1 NZ_CP050682:135285-135767 [Klebsiella variicola]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQIHERTLFLKLP

Nucleotide

• Length: 483 bp

>T153092 NZ_CP066756:695300-695614 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT153092 WP_003026799.1 NZ_CP050682:135031-135297 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT153092 NZ_CP066756:695611-696027 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66