Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | stbDE/ParE-YafN |
| Location | 325093..325615 | Replicon | plasmid pNDM-IMP-1 |
| Accession | NZ_CP050681 | ||
| Organism | Klebsiella variicola strain SHET-01 | ||
Toxin (Protein)
| Gene name | stbE | Uniprot ID | A0A7H0EVH6 |
| Locus tag | HCO75_RS28280 | Protein ID | WP_014839901.1 |
| Coordinates | 325093..325377 (-) | Length | 95 a.a. |
Antitoxin (Protein)
| Gene name | stbD | Uniprot ID | A0A7H0EVH5 |
| Locus tag | HCO75_RS28285 | Protein ID | WP_014839900.1 |
| Coordinates | 325367..325615 (-) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HCO75_RS28240 (HCO75_28530) | 320349..320558 | + | 210 | WP_125316032.1 | hypothetical protein | - |
| HCO75_RS28245 (HCO75_28535) | 320621..321565 | + | 945 | WP_014839903.1 | DsbA family protein | - |
| HCO75_RS28250 (HCO75_28540) | 321646..322167 | + | 522 | WP_048336588.1 | hypothetical protein | - |
| HCO75_RS28255 (HCO75_28545) | 322164..322766 | + | 603 | WP_014839902.1 | HAD domain-containing protein | - |
| HCO75_RS28260 | 323007..323402 | + | 396 | Protein_347 | cation:proton antiporter | - |
| HCO75_RS28265 | 323424..323726 | + | 303 | WP_233337821.1 | DUF2807 domain-containing protein | - |
| HCO75_RS28270 (HCO75_28555) | 323743..324420 | - | 678 | WP_042934501.1 | hypothetical protein | - |
| HCO75_RS28275 (HCO75_28560) | 324426..324902 | - | 477 | WP_042934526.1 | hypothetical protein | - |
| HCO75_RS28280 (HCO75_28565) | 325093..325377 | - | 285 | WP_014839901.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HCO75_RS28285 (HCO75_28570) | 325367..325615 | - | 249 | WP_014839900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| HCO75_RS28290 (HCO75_28575) | 325956..327359 | + | 1404 | WP_001567368.1 | ISNCY-like element ISKpn21 family transposase | - |
| HCO75_RS28295 (HCO75_28580) | 327388..328020 | - | 633 | WP_001567369.1 | hypothetical protein | - |
| HCO75_RS28300 (HCO75_28585) | 328565..329173 | - | 609 | WP_024266306.1 | DUF2913 family protein | - |
| HCO75_RS28305 (HCO75_28590) | 329310..329708 | - | 399 | WP_014839899.1 | H-NS family nucleoid-associated regulatory protein | - |
| HCO75_RS28310 (HCO75_28595) | 330082..330438 | - | 357 | WP_017901071.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mph(E) / msr(E) / sul1 / qacE / aadA16 / dfrA27 / blaNDM-1 / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / blaSFO-1 / blaIMP-4 | - | 1..347317 | 347317 | |
| - | flank | IS/Tn | - | - | 325956..327359 | 1403 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 10866.46 Da Isoelectric Point: 10.5831
>T153091 WP_014839901.1 NZ_CP050681:c325377-325093 [Klebsiella variicola]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN
Download Length: 285 bp
>T153091 NZ_CP066756:c651501-651037 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 9071.29 Da Isoelectric Point: 3.8832
>AT153091 WP_014839900.1 NZ_CP050681:c325615-325367 [Klebsiella variicola]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL
Download Length: 249 bp
>AT153091 NZ_CP066756:c651836-651501 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7H0EVH6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7H0EVH5 |