Detailed information of TA system TA153090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5430524..5431149
• Replicon: chromosome
• Accession: NZ_CP050680
• Organism: Klebsiella variicola strain SHET-01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1F2M041
• Locus tag: HCO75_RS26160
• Protein ID: WP_008807903.1
• Coordinates: 5430524..5430907 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: HCO75_RS26165
• Protein ID: WP_004150355.1
• Coordinates: 5430907..5431149 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCO75_RS26145 (HCO75_26420)	5427891..5428793	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HCO75_RS26150 (HCO75_26425)	5428790..5429425	+	636	WP_008807902.1	formate dehydrogenase cytochrome b556 subunit	-
HCO75_RS26155 (HCO75_26430)	5429422..5430351	+	930	WP_039102683.1	formate dehydrogenase accessory protein FdhE	-
HCO75_RS26160 (HCO75_26435)	5430524..5430907	-	384	WP_008807903.1	type II toxin-antitoxin system VapC family toxin	Toxin
HCO75_RS26165 (HCO75_26440)	5430907..5431149	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
HCO75_RS26170 (HCO75_26445)	5431354..5432271	+	918	WP_039102682.1	alpha/beta hydrolase	-
HCO75_RS26175 (HCO75_26450)	5432286..5433227	-	942	WP_012543287.1	fatty acid biosynthesis protein FabY	-
HCO75_RS26180 (HCO75_26455)	5433272..5433709	-	438	WP_039102681.1	D-aminoacyl-tRNA deacylase	-
HCO75_RS26185 (HCO75_26460)	5433706..5434566	-	861	WP_008807907.1	virulence factor BrkB family protein	-
HCO75_RS26190 (HCO75_26465)	5434560..5435159	-	600	WP_008807908.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T153090 WP_008807903.1 NZ_CP050680:c5430907-5430524 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Nucleotide

• Length: 384 bp

>T153090 NZ_CP066756:c401152-400667 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT153090 WP_004150355.1 NZ_CP050680:c5431149-5430907 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT153090 NZ_CP066756:c401421-401143 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A1F2M041

Antitoxin

• AlphaFold DB: A0A0H3GGU9