Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5430524..5431149 Replicon chromosome
Accession NZ_CP050680
Organism Klebsiella variicola strain SHET-01

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag HCO75_RS26160 Protein ID WP_008807903.1
Coordinates 5430524..5430907 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HCO75_RS26165 Protein ID WP_004150355.1
Coordinates 5430907..5431149 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCO75_RS26145 (HCO75_26420) 5427891..5428793 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HCO75_RS26150 (HCO75_26425) 5428790..5429425 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
HCO75_RS26155 (HCO75_26430) 5429422..5430351 + 930 WP_039102683.1 formate dehydrogenase accessory protein FdhE -
HCO75_RS26160 (HCO75_26435) 5430524..5430907 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
HCO75_RS26165 (HCO75_26440) 5430907..5431149 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HCO75_RS26170 (HCO75_26445) 5431354..5432271 + 918 WP_039102682.1 alpha/beta hydrolase -
HCO75_RS26175 (HCO75_26450) 5432286..5433227 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
HCO75_RS26180 (HCO75_26455) 5433272..5433709 - 438 WP_039102681.1 D-aminoacyl-tRNA deacylase -
HCO75_RS26185 (HCO75_26460) 5433706..5434566 - 861 WP_008807907.1 virulence factor BrkB family protein -
HCO75_RS26190 (HCO75_26465) 5434560..5435159 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153090 WP_008807903.1 NZ_CP050680:c5430907-5430524 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T153090 NZ_CP066756:c401152-400667 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153090 WP_004150355.1 NZ_CP050680:c5431149-5430907 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153090 NZ_CP066756:c401421-401143 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References