Detailed information of TA system TA153089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5105874..5107555
• Replicon: chromosome
• Accession: NZ_CP050680
• Organism: Klebsiella variicola strain SHET-01

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A7H4S924
• Locus tag: HCO75_RS24665
• Protein ID: WP_012969143.1
• Coordinates: 5105874..5106791 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: HCO75_RS24670
• Protein ID: WP_039102729.1
• Coordinates: 5106797..5107555 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCO75_RS24645 (HCO75_24890)	5101180..5102700	+	1521	WP_008807253.1	sugar ABC transporter ATP-binding protein	-
HCO75_RS24650 (HCO75_24895)	5102697..5103695	+	999	WP_004206363.1	ribose ABC transporter permease	-
HCO75_RS24655 (HCO75_24900)	5103726..5104667	+	942	WP_012543155.1	ABC transporter substrate-binding protein	-
HCO75_RS24660 (HCO75_24905)	5104742..5105749	+	1008	WP_039102730.1	carbohydrate kinase family protein	-
HCO75_RS24665 (HCO75_24910)	5105874..5106791	-	918	WP_012969143.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HCO75_RS24670 (HCO75_24915)	5106797..5107555	-	759	WP_039102729.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HCO75_RS24675 (HCO75_24920)	5107641..5108369	-	729	WP_008807258.1	response regulator transcription factor	-
HCO75_RS24680 (HCO75_24925)	5108642..5110789	+	2148	WP_077598880.1	formate dehydrogenase subunit alpha	-
HCO75_RS24685 (HCO75_24930)	5111055..5111741	+	687	WP_016160120.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34721.11 Da
• Isoelectric Point: 5.4707

>T153089 WP_012969143.1 NZ_CP050680:c5106791-5105874 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMNAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T153089 NZ_CP066756:375549-376523 [Escherichia coli O157:H7]
GTGGGGCGTGCCGGTCGCGGTATCGATTCTGCTGTTGGCATACTGCTTTGCGGCAGTGAAGACCGTGCTATTCATAAATT
CTTCCGTGAAAGCGCCTTTCCTGCGGAGGCGCAAATTCATGAAATACTCAACGTACTTAGCGTGAATGACGGTCTTACCT
TACGAGGCATTGAACAACGGACGAATCTCCGTTATGGGCAAATAGAAAAAGCACTGAAATTATTGGTAGCTGAAAATCCA
TCGCCTGTGGTGTATACCGAGAAATTATGGCGCAGAACAATTGTCAGTTTTTCTCCTGACCATGAACGAATTAACCATTT
GATGAATCAGAGAAAAAGTGAGCTGGCAGACGTTGAAAGCTATATCACGACCAAGGAGTGCAAAATGCAATTTCTGCGCC
GCGCACTCGATGAGCCAGGTGCCGAACATTGTGGTAAATGTAGCAGTTGTCTTCAGCATCCGTTGTTGTCGCCCGACATT
GATAGCGGCTTACTCCATGCGGCAAACTTATTTATTAAACACGCTGACCTGTCATTAAATCTCAATAAACAGGTGGCAGC
TGGGGCTTTTACGCAATACGGATTTAAAGGGAACTTGCCTGCGAGTTTACAAGGATCTACGGGGCGAATCCTTTCTCGTT
GGGGATATTCCGGGTGGGGAAAGCAGGTAGCACAGGAGAAAAAAACGGGGCGATTCAGTGATGAACTGGTGGAGGCATGT
GCGGAAATGGTTCGCCAACGCTGGAATCCGCATCCTGAACCAACCTGGGTATGCTGCGTTCCTTCATTAAAGCACCTCGA
CCTGGTTCCTGATTTTGAAAGCACCTCGACCTGGTTCCTGATTTTGCCCGGCGACTGGCGGCGAAACTTGGCTTACCTTT
TATTGATGCCATTGAAAAAGTCGTGGACAATCCACCGCAGAAAATGCAGCAAAACCGTTTCCACCAGTGTCAAAATCTCG
ACGGGGCGTTTGTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28642.97 Da
• Isoelectric Point: 10.1988

>AT153089 WP_039102729.1 NZ_CP050680:c5107555-5106797 [Klebsiella variicola]
MSSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVTLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT153089 NZ_CP066756:376582-377877 [Escherichia coli O157:H7]
ATGGACGCTGACAGTTCTAACAGCACTGTTACGCCAGGCAGGTTGCCCGACGGTTTATCCTCTTGCCCTTGCTTCTACCT
CGGTAAAAAATTGATGAATCTTTCAGCCAATGCACAAGCAACTCTCCTGCTAACCAGCGATTTTTCTCGCGCGGCGGCGA
GTAAGTATAAACCTCTTAGTAATAGTGAATGGGGGAAGTTTGCATTATGGCTGAAGCACCAACGTATCAGTCCCGCCGAG
CTTCTGGTGCCGCAACCGCAAGAGAAACTTACAGGCTGGAGCGATCCGCGTATTTCTCAGGAGCGTATTCTTGGCTTGCT
GGCGCGTGGTCATAGTCTGGCGTTGGCGGTAGATAAGTGGCAACGCGCCGGTTTATGGATCTTAACCCGCGGAGATGCTG
ATTATCCCGTTCGCTTGAAAAACCGATTGCGAACGGATGCACCTCCCGTTTTATTTGGCTGCGGGAATAAAGCATTACTG
CAAGCGGAAGGTATGGCGATTGTTGGCTCGCGAGATGCTCCGACTGACGATTTGCGCTATACCCAACAACTGGCCGCGAA
ACTGGCCCAACAGGGGATTTGCGTTATCTCTGGTGGTGCGCGAGGTATTGATGAATGTGCAATGGCGTCGGCACTGGAGG
CCGGGGGAACTGCCGTTGGCGTATTAGCTGATAGCTTGTTAAAAACGAGTACGTTAGTGAAATGGCGTGAAGGGCTTATA
GCAGGCAACCTGGTGTTGATTTCGCCGTTTTACCCAGAGGTACGTTTCACCGTCGGCAATGCGATGGCGCGAAATAAATA
TATTTATTGCCTTGCTGAAAGCGCAATGGTTGTACGTGCGGGAATGACCGGTGGAACGATAACCGGGGCGATGGAGGCAT
TAAAACATCAGTGGCTGCCTGTGCAGGTTAAACCAAATCAGGATATGCAATCAGCCAATTCACGATTAGTAGAAAATGGG
GCGTCATGGAGTGCTGAACAGGCTGAGAATGTGACGATCAGACTGCCAGACGTTCCTGGGCTGATGTATGACAGAGCACT
CCGTAACGCTCAACCAGAACTGTTTTCGCTGCATGAAGATGACGCAAATTACGCAGTAATGCCCGCGTATACGCCTGTCG
ATTTTTATCAACTCTTTGTGGCGGAACTGGCGATCCTTGCAAAGGAATCGATAAGTATTGAAAGGCTGGCGTCTTGTACT
GGTTTAACCATCGAACAAATTAGTGTGTGGCTGAACCGCGCAGAAGAAGAGGGAAGGGTTATCCGATTGGGCGAAGGTCA
TTATCAGTTCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7H4S924