153088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4215448..4216067 Replicon chromosome
Accession NZ_CP050680
Organism Klebsiella variicola strain SHET-01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HCO75_RS20425 Protein ID WP_002892050.1
Coordinates 4215849..4216067 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag HCO75_RS20420 Protein ID WP_008805436.1
Coordinates 4215448..4215822 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCO75_RS20410 (HCO75_20615) 4210603..4211796 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HCO75_RS20415 (HCO75_20620) 4211819..4214965 + 3147 WP_012968813.1 multidrug efflux RND transporter permease subunit AcrB -
HCO75_RS20420 (HCO75_20625) 4215448..4215822 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
HCO75_RS20425 (HCO75_20630) 4215849..4216067 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HCO75_RS20430 (HCO75_20635) 4216226..4216792 + 567 WP_039102911.1 maltose O-acetyltransferase -
HCO75_RS20435 (HCO75_20640) 4216764..4216892 - 129 Protein_4014 hypothetical protein -
HCO75_RS20440 (HCO75_20645) 4216929..4217399 + 471 WP_008805434.1 YlaC family protein -
HCO75_RS20445 (HCO75_20650) 4217368..4218825 - 1458 WP_039102910.1 PLP-dependent aminotransferase family protein -
HCO75_RS20450 (HCO75_20655) 4218926..4219624 + 699 WP_016160391.1 GNAT family protein -
HCO75_RS20455 (HCO75_20660) 4219621..4219761 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HCO75_RS20460 (HCO75_20665) 4219761..4220024 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T153088 WP_002892050.1 NZ_CP050680:4215849-4216067 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T153088 NZ_CP066756:352010-352537 [Escherichia coli O157:H7]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT153088 WP_008805436.1 NZ_CP050680:4215448-4215822 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT153088 NZ_CP066756:351737-352003 [Escherichia coli O157:H7]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References