Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1868998..1869588 Replicon chromosome
Accession NZ_CP050680
Organism Klebsiella variicola strain SHET-01

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag HCO75_RS09060 Protein ID WP_039104087.1
Coordinates 1869256..1869588 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag HCO75_RS09055 Protein ID WP_039104089.1
Coordinates 1868998..1869255 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCO75_RS09035 (HCO75_09140) 1864607..1865182 + 576 WP_016161434.1 hypothetical protein -
HCO75_RS09040 (HCO75_09145) 1865359..1866195 + 837 WP_008804155.1 alpha/beta hydrolase -
HCO75_RS09045 (HCO75_09150) 1866402..1867373 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
HCO75_RS09050 (HCO75_09155) 1867370..1868470 - 1101 WP_039104091.1 AarF/UbiB family protein -
HCO75_RS09055 (HCO75_09160) 1868998..1869255 + 258 WP_039104089.1 antitoxin Antitoxin
HCO75_RS09060 (HCO75_09165) 1869256..1869588 + 333 WP_039104087.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HCO75_RS09070 (HCO75_09180) 1869911..1871347 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HCO75_RS09080 (HCO75_09190) 1871720..1873174 - 1455 WP_008804170.1 AMP nucleosidase -
HCO75_RS09085 (HCO75_09195) 1873305..1873550 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11895.77 Da        Isoelectric Point: 10.1839

>T153078 WP_039104087.1 NZ_CP050680:1869256-1869588 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAIINEVLARLDAILS

Download         Length: 333 bp

>T153078 NZ_CP066753:4826943-4827284 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9432.97 Da        Isoelectric Point: 4.4915

>AT153078 WP_039104089.1 NZ_CP050680:1868998-1869255 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT153078 NZ_CP066753:c4826948-4826872 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References