Detailed information of TA system TA153078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1868998..1869588
• Replicon: chromosome
• Accession: NZ_CP050680
• Organism: Klebsiella variicola strain SHET-01

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HCO75_RS09060
• Protein ID: WP_039104087.1
• Coordinates: 1869256..1869588 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HCO75_RS09055
• Protein ID: WP_039104089.1
• Coordinates: 1868998..1869255 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCO75_RS09035 (HCO75_09140)	1864607..1865182	+	576	WP_016161434.1	hypothetical protein	-
HCO75_RS09040 (HCO75_09145)	1865359..1866195	+	837	WP_008804155.1	alpha/beta hydrolase	-
HCO75_RS09045 (HCO75_09150)	1866402..1867373	+	972	WP_016161433.1	sensor domain-containing diguanylate cyclase	-
HCO75_RS09050 (HCO75_09155)	1867370..1868470	-	1101	WP_039104091.1	AarF/UbiB family protein	-
HCO75_RS09055 (HCO75_09160)	1868998..1869255	+	258	WP_039104089.1	antitoxin	Antitoxin
HCO75_RS09060 (HCO75_09165)	1869256..1869588	+	333	WP_039104087.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HCO75_RS09070 (HCO75_09180)	1869911..1871347	+	1437	WP_016161429.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HCO75_RS09080 (HCO75_09190)	1871720..1873174	-	1455	WP_008804170.1	AMP nucleosidase	-
HCO75_RS09085 (HCO75_09195)	1873305..1873550	-	246	WP_008804171.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11895.77 Da
• Isoelectric Point: 10.1839

>T153078 WP_039104087.1 NZ_CP050680:1869256-1869588 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAIINEVLARLDAILS

Nucleotide

• Length: 333 bp

>T153078 NZ_CP066753:4826943-4827284 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9432.97 Da
• Isoelectric Point: 4.4915

>AT153078 WP_039104089.1 NZ_CP050680:1868998-1869255 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT153078 NZ_CP066753:c4826948-4826872 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT