Detailed information of TA system TA153077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1679739..1680724
• Replicon: chromosome
• Accession: NZ_CP050680
• Organism: Klebsiella variicola strain SHET-01

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HCO75_RS08260
• Protein ID: WP_274757448.1
• Coordinates: 1680182..1680724 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MDT5
• Locus tag: HCO75_RS08255
• Protein ID: WP_012541033.1
• Coordinates: 1679739..1680185 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCO75_RS08240 (HCO75_08350)	1676034..1677032	+	999	WP_008804028.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
HCO75_RS08245 (HCO75_08355)	1677151..1678671	+	1521	WP_008804029.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
HCO75_RS08250 (HCO75_08360)	1678687..1679697	+	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
HCO75_RS08255 (HCO75_08365)	1679739..1680185	-	447	WP_012541033.1	helix-turn-helix transcriptional regulator	Antitoxin
HCO75_RS08260	1680182..1680724	-	543	WP_274757448.1	HipA domain-containing protein	Toxin
HCO75_RS08265	1680766..1681287	-	522	WP_022064813.1	hypothetical protein	-
HCO75_RS08270 (HCO75_08375)	1681402..1682133	-	732	WP_008804033.1	outer membrane permeability protein SanA	-
HCO75_RS08275 (HCO75_08380)	1682426..1683310	-	885	WP_274757449.1	cytidine deaminase	-
HCO75_RS08280 (HCO75_08385)	1683439..1684134	-	696	WP_008804035.1	CidB/LrgB family autolysis modulator	-
HCO75_RS08285 (HCO75_08390)	1684124..1684528	-	405	WP_004201620.1	CidA/LrgA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (45-143)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 20346.51 Da
• Isoelectric Point: 5.2649

>T153077 WP_274757448.1 NZ_CP050680:c1680724-1680182 [Klebsiella variicola]
MESVYSLLQKAPATILDYETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKADNEI
TLAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSHLKVRGVPVQIL
EMPSIGFKFIPDKLARWRLL

Nucleotide

• Length: 543 bp

>T153077 NZ_CP066753:c4711090-4710776 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16396.05 Da
• Isoelectric Point: 10.5417

>AT153077 WP_012541033.1 NZ_CP050680:c1680185-1679739 [Klebsiella variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT153077 NZ_CP066753:c4711326-4711093 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1F2MDT5