Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 793653..794310 Replicon chromosome
Accession NZ_CP050680
Organism Klebsiella variicola strain SHET-01

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag HCO75_RS03905 Protein ID WP_002916310.1
Coordinates 793900..794310 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag HCO75_RS03900 Protein ID WP_002916312.1
Coordinates 793653..793919 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCO75_RS03875 (HCO75_03925) 790095..790823 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
HCO75_RS03880 (HCO75_03930) 790874..791185 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
HCO75_RS03885 (HCO75_03935) 791349..792008 + 660 WP_008806429.1 hemolysin III family protein -
HCO75_RS03890 (HCO75_03940) 792039..792377 + 339 WP_145940792.1 hypothetical protein -
HCO75_RS03895 (HCO75_03945) 792424..793407 - 984 WP_012967246.1 tRNA-modifying protein YgfZ -
HCO75_RS03900 (HCO75_03950) 793653..793919 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
HCO75_RS03905 (HCO75_03955) 793900..794310 + 411 WP_002916310.1 protein YgfX Toxin
HCO75_RS03910 (HCO75_03960) 794317..794838 - 522 WP_008806427.1 flavodoxin FldB -
HCO75_RS03915 (HCO75_03965) 794939..795835 + 897 WP_039102537.1 site-specific tyrosine recombinase XerD -
HCO75_RS03920 (HCO75_03970) 795858..796571 + 714 WP_008806425.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HCO75_RS03925 (HCO75_03975) 796577..798310 + 1734 WP_039102536.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T153076 WP_002916310.1 NZ_CP050680:793900-794310 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T153076 NZ_CP066753:4479390-4479650 [Escherichia coli O157:H7]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT153076 WP_002916312.1 NZ_CP050680:793653-793919 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153076 NZ_CP066753:4479111-4479371 [Escherichia coli O157:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References