Detailed information of TA system TA153075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 322645..323231
• Replicon: chromosome
• Accession: NZ_CP050680
• Organism: Klebsiella variicola strain SHET-01

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A2W7TCK5
• Locus tag: HCO75_RS01475
• Protein ID: WP_008806973.1
• Coordinates: 322863..323231 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: HCO75_RS01470
• Protein ID: WP_004174006.1
• Coordinates: 322645..322866 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCO75_RS01450 (HCO75_01460)	318802..319728	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HCO75_RS01455 (HCO75_01465)	319725..321002	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
HCO75_RS01460 (HCO75_01470)	320999..321766	+	768	WP_012967117.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HCO75_RS01465 (HCO75_01475)	321768..322481	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HCO75_RS01470 (HCO75_01480)	322645..322866	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HCO75_RS01475 (HCO75_01485)	322863..323231	+	369	WP_008806973.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HCO75_RS01480 (HCO75_01490)	323508..324824	+	1317	WP_022066271.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HCO75_RS01485 (HCO75_01495)	324931..325818	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HCO75_RS01490 (HCO75_01500)	325815..326660	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HCO75_RS01495 (HCO75_01505)	326662..327732	+	1071	WP_023323353.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	317631..331378	13747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13639.01 Da
• Isoelectric Point: 7.3191

>T153075 WP_008806973.1 NZ_CP050680:322863-323231 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLVISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T153075 NZ_CP066753:c4473391-4472993 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT153075 WP_004174006.1 NZ_CP050680:322645-322866 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT153075 NZ_CP066753:c4473687-4473394 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A2W7TCK5

Antitoxin

• AlphaFold DB: A0A5E5YJY7