Detailed information of TA system TA153073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/-
• Location: 1394142..1394842
• Replicon: chromosome
• Accession: NZ_CP050650
• Organism: Enterococcus faecium strain BIOPOP-3 WT

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: HC354_RS06725
• Protein ID: WP_119364934.1
• Coordinates: 1394142..1394567 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: HC354_RS06730
• Protein ID: WP_002313665.1
• Coordinates: 1394585..1394842 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC354_RS06695	1389223..1389666	+	444	WP_002345979.1	hypothetical protein	-
HC354_RS06700	1389875..1390465	-	591	WP_002308925.1	glutamine amidotransferase	-
HC354_RS06705	1391190..1391858	+	669	WP_002342166.1	zeta toxin family protein	-
HC354_RS06710	1391851..1392012	+	162	WP_002342167.1	hypothetical protein	-
HC354_RS06715	1392222..1392653	+	432	WP_002342168.1	N-6 DNA methylase	-
HC354_RS06720	1393152..1393910	+	759	WP_167779246.1	restriction endonuclease	-
HC354_RS06725	1394142..1394567	-	426	WP_119364934.1	toxin	Toxin
HC354_RS06730	1394585..1394842	-	258	WP_002313665.1	hypothetical protein	Antitoxin
HC354_RS06735	1394986..1395945	+	960	WP_002301399.1	IS30 family transposase	-
HC354_RS13600	1396109..1396411	+	303	Protein_1290	hypothetical protein	-
HC354_RS13605	1396427..1396763	+	337	Protein_1291	hypothetical protein	-
HC354_RS06745	1397239..1397469	+	231	WP_002292139.1	hypothetical protein	-
HC354_RS06750	1397466..1397732	+	267	WP_002292138.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HC354_RS06755	1397984..1398211	+	228	Protein_1294	hypothetical protein	-
HC354_RS06760	1398227..1399393	+	1167	WP_167779247.1	oxygen-independent coproporphyrinogen III oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1394986..1395945	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16705.81 Da
• Isoelectric Point: 4.6731

>T153073 WP_119364934.1 NZ_CP050650:c1394567-1394142 [Enterococcus faecium]
MNVISEGLYFEAADLANELSEEVSYYCSKKMEHVKCYDIENYVNEVEDVEFIDYDFKDNLSKKMLGSITKVHGEIIITTN
QHLMLERKNFTKMHEIIHYYKDVPYLGESHAFSDMILEDTYLPEDYKKTSSKYRCQYPHGE

Nucleotide

• Length: 426 bp

>T153073 NZ_CP066753:3733301-3733687 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9913.09 Da
• Isoelectric Point: 4.2290

>AT153073 WP_002313665.1 NZ_CP050650:c1394842-1394585 [Enterococcus faecium]
MLSNYQLVTVIADFFNVSTDFLLNRTSDPTPPKRSEEDQLGDSILSHFRLNTADMEVEDIEELEEELNEYMDFLVQRAKA
KKNKK

Nucleotide

• Length: 258 bp

>AT153073 NZ_CP066753:3733677-3734006 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA