Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 773631..774202 Replicon chromosome
Accession NZ_CP050648
Organism Enterococcus faecium strain BIOPOP-3 ALE

Toxin (Protein)


Gene name mazF Uniprot ID R2Q2D5
Locus tag HC353_RS03810 Protein ID WP_002341043.1
Coordinates 773861..774202 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag HC353_RS03805 Protein ID WP_025477367.1
Coordinates 773631..773861 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC353_RS03785 769895..770488 + 594 WP_002329652.1 malonate decarboxylase holo-ACP synthase -
HC353_RS03790 770512..771597 + 1086 WP_002329651.1 acyltransferase -
HC353_RS03795 771619..772488 + 870 WP_002329650.1 triphosphoribosyl-dephospho-CoA synthase MdcB -
HC353_RS03800 772600..773364 + 765 Protein_712 TIM44-like domain-containing protein -
HC353_RS03805 773631..773861 + 231 WP_025477367.1 hypothetical protein Antitoxin
HC353_RS03810 773861..774202 + 342 WP_002341043.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HC353_RS03815 774918..777020 + 2103 WP_167779057.1 PTS sugar transporter subunit IIA -
HC353_RS03820 777056..777358 + 303 Protein_716 PTS sugar transporter subunit IIB -
HC353_RS03825 777384..778673 + 1290 WP_167779058.1 PTS sugar transporter subunit IIC -
HC353_RS03830 778692..779099 + 408 Protein_718 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13316.66 Da        Isoelectric Point: 9.9258

>T153069 WP_002341043.1 NZ_CP050648:773861-774202 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T153069 NZ_CP066753:3083575-3083682 [Escherichia coli O157:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT153069 WP_025477367.1 NZ_CP050648:773631-773861 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT153069 NZ_CP066753:c3083527-3083468 [Escherichia coli O157:H7]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829AD04

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References