Detailed information of TA system TA153065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 38035..38570
• Replicon: plasmid pIncFIB
• Accession: NZ_CP050647
• Organism: Escherichia coli strain PapM-36-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G6008_RS22785
• Protein ID: WP_089584420.1
• Coordinates: 38035..38322 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: G6008_RS22790
• Protein ID: WP_001139206.1
• Coordinates: 38319..38570 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6008_RS22770	33677..37174	+	3498	WP_167778855.1	autotransporter outer membrane beta-barrel domain-containing protein	-
G6008_RS22775	37349..37554	-	206	Protein_35	transposase	-
G6008_RS22780	37544..37747	+	204	WP_167778856.1	alkaline phosphatase	-
G6008_RS22785	38035..38322	-	288	WP_089584420.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6008_RS22790	38319..38570	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6008_RS22795	39524..40381	-	858	WP_167778857.1	incFII family plasmid replication initiator RepA	-
G6008_RS22800	40374..40448	-	75	WP_001365705.1	RepA leader peptide Tap	-
G6008_RS22805	40695..40943	-	249	WP_000083838.1	replication regulatory protein RepA	-
G6008_RS22810	41227..41433	-	207	WP_001553767.1	type I toxin-antitoxin system Hok family toxin	-
-	41420..41481	+	62	NuclAT_1	-	-
-	41420..41481	+	62	NuclAT_1	-	-
-	41420..41481	+	62	NuclAT_1	-	-
-	41420..41481	+	62	NuclAT_1	-	-
G6008_RS22815	42141..42602	-	462	WP_089572206.1	thermonuclease family protein	-
G6008_RS22820	43346..43549	-	204	WP_089571986.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	paa / papC / papH	1..107449	107449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11144.19 Da
• Isoelectric Point: 10.6261

>T153065 WP_089584420.1 NZ_CP050647:c38322-38035 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASARLRGLKDCYKIKLRTSGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T153065 NZ_CP066753:2281334-2281437 [Escherichia coli O157:H7]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT153065 WP_001139206.1 NZ_CP050647:c38570-38319 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT153065 NZ_CP066753:c2281475-2281204 [Escherichia coli O157:H7]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: T1PXV2