Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3901524..3902178 Replicon chromosome
Accession NZ_CP050646
Organism Escherichia coli strain PapM-36-2

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag G6008_RS18675 Protein ID WP_000244772.1
Coordinates 3901771..3902178 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G6008_RS18670 Protein ID WP_000354046.1
Coordinates 3901524..3901790 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6008_RS18645 3896693..3897436 + 744 WP_000951964.1 SDR family oxidoreductase -
G6008_RS18650 3897493..3898926 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
G6008_RS18655 3898971..3899282 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G6008_RS18660 3899446..3900105 + 660 WP_000250274.1 hemolysin III family protein -
G6008_RS18665 3900301..3901281 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G6008_RS18670 3901524..3901790 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G6008_RS18675 3901771..3902178 + 408 WP_000244772.1 protein YgfX Toxin
G6008_RS18680 3902218..3902739 - 522 WP_001055874.1 flavodoxin FldB -
G6008_RS18685 3902851..3903747 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G6008_RS18690 3903772..3904482 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6008_RS18695 3904488..3906221 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T153062 WP_000244772.1 NZ_CP050646:3901771-3902178 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T153062 NZ_CP066753:712775-713089 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT153062 WP_000354046.1 NZ_CP050646:3901524-3901790 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153062 NZ_CP066753:713086-713502 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References