Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 3318321..3318952 Replicon chromosome
Accession NZ_CP050646
Organism Escherichia coli strain PapM-36-2

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag G6008_RS15755 Protein ID WP_001259385.1
Coordinates 3318321..3318596 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag G6008_RS15760 Protein ID WP_000593555.1
Coordinates 3318593..3318952 (+) Length 120 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6008_RS15740 3313325..3314392 + 1068 WP_000361467.1 HlyD family secretion protein -
G6008_RS15745 3314389..3317124 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
G6008_RS15750 3317124..3318248 + 1125 WP_001314210.1 ABC transporter permease -
G6008_RS15755 3318321..3318596 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
G6008_RS15760 3318593..3318952 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
G6008_RS15765 3319072..3319473 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
G6008_RS15770 3319479..3320285 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
G6008_RS15775 3320282..3321046 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
G6008_RS15780 3321046..3321879 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
G6008_RS15785 3321876..3322820 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3310704..3318952 8248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(30-80)

Antitoxin

(60-112)


Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T153060 WP_001259385.1 NZ_CP050646:3318321-3318596 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T153060 NZ_CP066753:c417314-416829 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT153060 WP_000593555.1 NZ_CP050646:3318593-3318952 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT153060 NZ_CP066753:c417583-417305 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References