Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2470428..2471023 Replicon chromosome
Accession NZ_CP050646
Organism Escherichia coli strain PapM-36-2

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag G6008_RS11860 Protein ID WP_000239579.1
Coordinates 2470428..2470778 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag G6008_RS11865 Protein ID WP_001223208.1
Coordinates 2470772..2471023 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6008_RS11840 2465704..2466726 - 1023 WP_001296689.1 ABC transporter permease -
G6008_RS11845 2466740..2468242 - 1503 WP_000205794.1 sugar ABC transporter ATP-binding protein -
G6008_RS11850 2468552..2469508 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
G6008_RS11855 2469818..2470348 + 531 WP_000055072.1 inorganic diphosphatase -
G6008_RS11860 2470428..2470778 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
G6008_RS11865 2470772..2471023 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
G6008_RS11870 2471235..2471576 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
G6008_RS11875 2471579..2475358 - 3780 WP_000060895.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T153055 WP_000239579.1 NZ_CP050646:c2470778-2470428 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T153055 NZ_CP066750:c59382-59077 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT153055 WP_001223208.1 NZ_CP050646:c2471023-2470772 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT153055 NZ_CP066750:c59602-59384 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References