Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 724931..725569 Replicon chromosome
Accession NZ_CP050646
Organism Escherichia coli strain PapM-36-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G6008_RS03590 Protein ID WP_000813794.1
Coordinates 725393..725569 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G6008_RS03585 Protein ID WP_001270286.1
Coordinates 724931..725347 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6008_RS03565 720083..721024 - 942 WP_001251315.1 ABC transporter permease -
G6008_RS03570 721025..722038 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
G6008_RS03575 722056..723201 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
G6008_RS03580 723446..724852 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
G6008_RS03585 724931..725347 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G6008_RS03590 725393..725569 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G6008_RS03595 725791..726021 + 231 WP_000494244.1 YncJ family protein -
G6008_RS03600 726113..728074 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G6008_RS03605 728147..728683 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
G6008_RS03610 728736..729950 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T153049 WP_000813794.1 NZ_CP050646:c725569-725393 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T153049 NZ_CP066748:c4917967-4917617 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT153049 WP_001270286.1 NZ_CP050646:c725347-724931 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT153049 NZ_CP066748:c4918212-4917961 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References