Detailed information of TA system TA153048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 628191..629779
• Replicon: chromosome
• Accession: NZ_CP050646
• Organism: Escherichia coli strain PapM-36-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: E0IX85
• Locus tag: G6008_RS03190
• Protein ID: WP_001125454.1
• Coordinates: 628457..629779 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: G6008_RS03185
• Protein ID: WP_001296726.1
• Coordinates: 628191..628457 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6008_RS03185	628191..628457	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
G6008_RS03190	628457..629779	+	1323	WP_001125454.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
G6008_RS03195	630091..630299	+	209	Protein_631	helix-turn-helix transcriptional regulator	-
G6008_RS03200	630731..631294	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
G6008_RS03205	631655..632365	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	628191..644279	16088

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49307.90 Da
• Isoelectric Point: 8.8753

>T153048 WP_001125454.1 NZ_CP050646:628457-629779 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T153048 NZ_CP066748:4783089-4783430 [Escherichia coli O157:H7]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT153048 WP_001296726.1 NZ_CP050646:628191-628457 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT153048 NZ_CP066748:c4783094-4783018 [Escherichia coli O157:H7]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: E0IX85