Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 511937..512573 Replicon chromosome
Accession NZ_CP050532
Organism Bacillus subtilis subsp. subtilis str. SMY

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HCN55_RS02590 Protein ID WP_003156187.1
Coordinates 512223..512573 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag HCN55_RS02585 Protein ID WP_003225183.1
Coordinates 511937..512218 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCN55_RS02565 508296..508895 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
HCN55_RS02570 508990..509355 + 366 WP_003234281.1 holo-ACP synthase -
HCN55_RS02575 509521..510537 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
HCN55_RS02580 510652..511821 + 1170 WP_003234284.1 alanine racemase -
HCN55_RS02585 511937..512218 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HCN55_RS02590 512223..512573 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HCN55_RS02595 512688..513512 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
HCN55_RS02600 513517..513882 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
HCN55_RS02605 513886..514287 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
HCN55_RS02610 514299..515306 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
HCN55_RS02615 515368..515697 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
HCN55_RS02620 515694..516176 + 483 WP_003234299.1 anti-sigma B factor RsbW -
HCN55_RS02625 516142..516930 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
HCN55_RS02630 516930..517529 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T153019 WP_003156187.1 NZ_CP050532:512223-512573 [Bacillus subtilis subsp. subtilis str. SMY]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T153019 NZ_CP066726:941705-942067 [Staphylococcus xylosus]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTACAAGGGTCTGAACAAGGGGGAATTAGACCCGTCGT
TATTATACAAAACGATACTGGTAACAAATATAGTCCTACAGTAATTGTAGCTGCAATTACTGGTAGGATTAATAAAGCTA
AAATACCTACACATGTAGAGATAGAAAAAAGTAAATATAAACTTGATAAAGATTCTGTTATATTGTTAGAACAAATCAGA
ACTTTAGATAAAAATAGGCTAAAGGAAAAATTAACATATCTTTCTGAGAAAAAAATGAAAGAAGTTAATATTGCTATTGA
TATAAGTTTAGGATTACATAATATTAGGAATCATAAATCCTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT153019 WP_003225183.1 NZ_CP050532:511937-512218 [Bacillus subtilis subsp. subtilis str. SMY]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT153019 NZ_CP066726:941538-941708 [Staphylococcus xylosus]
ATGGCAAGTTTTAATCAAAGTAGAAATCATAGTATTGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGACTTAAA
TCTCTCCCTTGCAGCAGAAGCCTTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACATTAGCTCTAAGAATA
ATAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References