Detailed information of TA system TA152999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 2347509..2348300
• Replicon: chromosome
• Accession: NZ_CP050520
• Organism: Enterobacter sp. DNB-S2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HB664_RS11080
• Protein ID: WP_258235888.1
• Coordinates: 2347785..2348300 (+)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: HB664_RS11075
• Protein ID: WP_008499829.1
• Coordinates: 2347509..2347778 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB664_RS11045 (HB664_11045)	2343136..2343579	-	444	WP_032658095.1	helix-turn-helix domain-containing protein	-
HB664_RS11050 (HB664_11050)	2343732..2344973	+	1242	WP_219940796.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
HB664_RS11055 (HB664_11055)	2345007..2345234	-	228	WP_008499832.1	YccJ family protein	-
HB664_RS11060 (HB664_11060)	2345255..2345851	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
HB664_RS11065 (HB664_11065)	2346242..2346412	+	171	WP_023311077.1	general stress protein	-
HB664_RS11070 (HB664_11070)	2346530..2347447	+	918	WP_219940797.1	DMT family transporter	-
HB664_RS11075 (HB664_11075)	2347509..2347778	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
HB664_RS11080 (HB664_11080)	2347785..2348300	+	516	WP_258235888.1	GNAT family N-acetyltransferase	Toxin
HB664_RS11085 (HB664_11085)	2348380..2349702	-	1323	WP_219940798.1	pyrimidine utilization transport protein G	-
HB664_RS11090 (HB664_11090)	2349724..2350218	-	495	WP_032658088.1	pyrimidine utilization flavin reductase protein F	-
HB664_RS11095 (HB664_11095)	2350228..2350818	-	591	WP_047061542.1	malonic semialdehyde reductase	-
HB664_RS11100 (HB664_11100)	2350828..2351628	-	801	WP_219940799.1	pyrimidine utilization protein D	-
HB664_RS11105 (HB664_11105)	2351636..2352022	-	387	WP_010429508.1	pyrimidine utilization protein C	-
HB664_RS11110 (HB664_11110)	2352034..2352723	-	690	WP_033145167.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 19511.31 Da
• Isoelectric Point: 7.3817

>T152999 WP_258235888.1 NZ_CP050520:2347785-2348300 [Enterobacter sp. DNB-S2]
VENLTIEMLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQR
RIPYINVPSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLF
FPTKSIERLFE

Nucleotide

• Length: 516 bp

>T152999 NZ_CP066715:215094-215360 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTCTTAATTAAGAATTCAGCCAAAAATGACTTGAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCATATTATGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT152999 WP_008499829.1 NZ_CP050520:2347509-2347778 [Enterobacter sp. DNB-S2]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT152999 NZ_CP066715:214837-215094 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATGGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Antitoxin

• AlphaFold DB: W7PG43