Detailed information of TA system TA152997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1298740..1299316
• Replicon: chromosome
• Accession: NZ_CP050520
• Organism: Enterobacter sp. DNB-S2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HB664_RS06110
• Protein ID: WP_219940491.1
• Coordinates: 1298740..1299027 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HB664_RS06115
• Protein ID: WP_214580031.1
• Coordinates: 1299014..1299316 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB664_RS06080 (HB664_06070)	1294064..1294231	+	168	Protein_1171	antibiotic biosynthesis monooxygenase	-
HB664_RS06085 (HB664_06075)	1294240..1294551	+	312	WP_258235879.1	winged helix-turn-helix domain-containing protein	-
HB664_RS06090 (HB664_06080)	1294701..1295171	+	471	WP_064673177.1	MarR family transcriptional regulator	-
HB664_RS06095 (HB664_06085)	1295168..1296235	+	1068	WP_219940811.1	HlyD family secretion protein	-
HB664_RS06100 (HB664_06090)	1296225..1297301	+	1077	WP_023310256.1	DUF2955 domain-containing protein	-
HB664_RS06105 (HB664_06095)	1297298..1298569	-	1272	WP_219940490.1	DUF445 domain-containing protein	-
HB664_RS06110 (HB664_06100)	1298740..1299027	+	288	WP_219940491.1	BrnT family toxin	Toxin
HB664_RS06115 (HB664_06105)	1299014..1299316	+	303	WP_214580031.1	BrnA antitoxin family protein	Antitoxin
HB664_RS06120 (HB664_06110)	1299347..1299985	-	639	WP_219940492.1	LysE family translocator	-
HB664_RS06125 (HB664_06115)	1300033..1300776	-	744	WP_143347570.1	AraC family transcriptional regulator	-
HB664_RS06130 (HB664_06120)	1300928..1302298	+	1371	WP_143347569.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
HB664_RS06135 (HB664_06125)	1302344..1302664	-	321	WP_143347568.1	hypothetical protein	-
HB664_RS06140 (HB664_06130)	1302664..1303209	-	546	WP_219940493.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11202.69 Da
• Isoelectric Point: 6.7609

>T152997 WP_219940491.1 NZ_CP050520:1298740-1299027 [Enterobacter sp. DNB-S2]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
VRKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T152997 NZ_CP066714:840713-840934 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11421.07 Da
• Isoelectric Point: 10.1522

>AT152997 WP_214580031.1 NZ_CP050520:1299014-1299316 [Enterobacter sp. DNB-S2]
MSMVKYKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT152997 NZ_CP066714:840413-840700 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG