Detailed information of TA system TA152996

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1265783..1266486
• Replicon: chromosome
• Accession: NZ_CP050520
• Organism: Enterobacter sp. DNB-S2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HB664_RS05950
• Protein ID: WP_049017155.1
• Coordinates: 1266145..1266486 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HB664_RS05945
• Protein ID: WP_219940480.1
• Coordinates: 1265783..1266124 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB664_RS05910 (HB664_05900)	1260932..1261807	+	876	WP_219940478.1	GTPase family protein	-
HB664_RS05915 (HB664_05905)	1262051..1262767	+	717	WP_048291793.1	WYL domain-containing protein	-
HB664_RS05920 (HB664_05910)	1262803..1263256	+	454	Protein_1139	hypothetical protein	-
HB664_RS05925 (HB664_05915)	1263328..1263801	+	474	WP_049017159.1	hypothetical protein	-
HB664_RS05930 (HB664_05920)	1263922..1264743	+	822	WP_049017158.1	DUF932 domain-containing protein	-
HB664_RS05935 (HB664_05925)	1264774..1265217	+	444	WP_047365977.1	antirestriction protein	-
HB664_RS05940 (HB664_05930)	1265230..1265772	+	543	WP_219940479.1	DNA repair protein RadC	-
HB664_RS05945 (HB664_05935)	1265783..1266124	+	342	WP_219940480.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HB664_RS05950 (HB664_05940)	1266145..1266486	+	342	WP_049017155.1	TA system toxin CbtA family protein	Toxin
HB664_RS05955 (HB664_05945)	1266745..1267752	+	1008	WP_049017154.1	restriction endonuclease	-
HB664_RS05960 (HB664_05950)	1267933..1268802	-	870	WP_219940481.1	HNH endonuclease	-
HB664_RS05965 (HB664_05955)	1269103..1269570	+	468	WP_080940379.1	SymE family type I addiction module toxin	-
HB664_RS05970 (HB664_05960)	1269743..1270000	-	258	WP_258235878.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1250908..1267752	16844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12724.66 Da
• Isoelectric Point: 6.4514

>T152996 WP_049017155.1 NZ_CP050520:1266145-1266486 [Enterobacter sp. DNB-S2]
MKTLPATTPQAATLCLSPVAVWQMLLACLLEQHYGLTLNDTPFSDETVIQEHIDAGITLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T152996 NZ_CP066714:215094-215360 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTCTTAATTAAGAATTCAGCCAAAAATGACTTGAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCATATTATGAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12810.58 Da
• Isoelectric Point: 7.0164

>AT152996 WP_219940480.1 NZ_CP050520:1265783-1266124 [Enterobacter sp. DNB-S2]
MKYTDSENDSFLKWGLNRNVTPCFVARLVQVGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT152996 NZ_CP066714:214837-215094 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATGGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA