Detailed information of TA system TA152995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 952350..952942
• Replicon: chromosome
• Accession: NZ_CP050520
• Organism: Enterobacter sp. DNB-S2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HB664_RS04530
• Protein ID: WP_033144376.1
• Coordinates: 952640..952942 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A808IFF3
• Locus tag: HB664_RS04525
• Protein ID: WP_033144375.1
• Coordinates: 952350..952637 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB664_RS04520 (HB664_04515)	950722..952353	+	1632	WP_219940408.1	Na/Pi cotransporter family protein	-
HB664_RS04525 (HB664_04520)	952350..952637	-	288	WP_033144375.1	putative addiction module antidote protein	Antitoxin
HB664_RS04530 (HB664_04525)	952640..952942	-	303	WP_033144376.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HB664_RS04535 (HB664_04530)	953140..954012	+	873	WP_023309993.1	23S rRNA pseudouridine(2604) synthase RluF	-
HB664_RS04540 (HB664_04535)	954013..954285	-	273	WP_023309994.1	DUF3811 domain-containing protein	-
HB664_RS04545 (HB664_04540)	954336..955280	-	945	WP_219940409.1	ketopantoate/pantoate/pantothenate transporter PanS	-
HB664_RS04550 (HB664_04545)	955374..956723	-	1350	WP_023309996.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11284.02 Da
• Isoelectric Point: 9.9325

>T152995 WP_033144376.1 NZ_CP050520:c952942-952640 [Enterobacter sp. DNB-S2]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNPNGRSE

Nucleotide

• Length: 303 bp

>T152995 NZ_CP066713:785674-786027 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10111.59 Da
• Isoelectric Point: 6.4596

>AT152995 WP_033144375.1 NZ_CP050520:c952637-952350 [Enterobacter sp. DNB-S2]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAGD

Nucleotide

• Length: 288 bp

>AT152995 NZ_CP066713:785503-785673 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A808IFF3