Detailed information of TA system TA152994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 618554..619170
• Replicon: chromosome
• Accession: NZ_CP050520
• Organism: Enterobacter sp. DNB-S2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HB664_RS03045
• Protein ID: WP_219940338.1
• Coordinates: 618554..618925 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: HB664_RS03050
• Protein ID: WP_023309753.1
• Coordinates: 618928..619170 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB664_RS03030 (HB664_03025)	616054..616956	+	903	WP_010436855.1	formate dehydrogenase subunit beta	-
HB664_RS03035 (HB664_03030)	616953..617588	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
HB664_RS03040 (HB664_03035)	617585..618514	+	930	WP_023309751.1	formate dehydrogenase accessory protein FdhE	-
HB664_RS03045 (HB664_03040)	618554..618925	-	372	WP_219940338.1	type II toxin-antitoxin system VapC family toxin	Toxin
HB664_RS03050 (HB664_03045)	618928..619170	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
HB664_RS03055 (HB664_03050)	619369..620289	+	921	WP_219940339.1	alpha/beta hydrolase	-
HB664_RS03060 (HB664_03055)	620298..621239	-	942	WP_039264191.1	fatty acid biosynthesis protein FabY	-
HB664_RS03065 (HB664_03060)	621284..621721	-	438	WP_039264190.1	D-aminoacyl-tRNA deacylase	-
HB664_RS03070 (HB664_03065)	621718..622599	-	882	WP_033146965.1	virulence factor BrkB family protein	-
HB664_RS03075 (HB664_03070)	622593..623192	-	600	WP_143347721.1	glucose-1-phosphatase	-
HB664_RS03080 (HB664_03075)	623281..623751	-	471	WP_069303437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13665.81 Da
• Isoelectric Point: 7.3604

>T152994 WP_219940338.1 NZ_CP050520:c618925-618554 [Enterobacter sp. DNB-S2]
MERMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMGGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T152994 NZ_CP066713:770888-771109 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT152994 WP_023309753.1 NZ_CP050520:c619170-618928 [Enterobacter sp. DNB-S2]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT152994 NZ_CP066713:770588-770875 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0