Detailed information of TA system TA152992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4931646..4932222
• Replicon: chromosome
• Accession: NZ_CP050508
• Organism: Raoultella terrigena strain JH01

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HCK03_RS23155
• Protein ID: WP_123552783.1
• Coordinates: 4931935..4932222 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HCK03_RS23150
• Protein ID: WP_148251187.1
• Coordinates: 4931646..4931948 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCK03_RS23125	4926727..4927791	-	1065	WP_041143889.1	DUF2955 domain-containing protein	-
HCK03_RS23130	4927781..4928848	-	1068	WP_041143888.1	HlyD family secretion protein	-
HCK03_RS23135	4928855..4929319	-	465	WP_115193641.1	MarR family transcriptional regulator	-
HCK03_RS23140	4929777..4929941	-	165	WP_071844881.1	DUF1127 domain-containing protein	-
HCK03_RS23145	4930119..4931531	+	1413	WP_115193643.1	PLP-dependent aminotransferase family protein	-
HCK03_RS23150	4931646..4931948	-	303	WP_148251187.1	BrnA antitoxin family protein	Antitoxin
HCK03_RS23155	4931935..4932222	-	288	WP_123552783.1	BrnT family toxin	Toxin
HCK03_RS23160	4932340..4932759	-	420	WP_115193646.1	FosA family fosfomycin resistance glutathione transferase	-
HCK03_RS23165	4932763..4933662	-	900	WP_167692242.1	LysR family transcriptional regulator	-
HCK03_RS23170	4933750..4934532	+	783	WP_167692243.1	NAD(P)H-dependent oxidoreductase	-
HCK03_RS23175	4934679..4935257	+	579	WP_041143880.1	TetR/AcrR family transcriptional regulator	-
HCK03_RS23180	4935302..4936117	+	816	WP_167692244.1	helix-turn-helix domain-containing protein	-
HCK03_RS23185	4936114..4936632	+	519	WP_041143878.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11016.38 Da
• Isoelectric Point: 6.8502

>T152992 WP_123552783.1 NZ_CP050508:c4932222-4931935 [Raoultella terrigena]
MPVEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSHQDRYENGEHRWQTIGLVHGIIVILVVHSVRFESGAEVIRIIS
ARKADGKERSRYEHS

Nucleotide

• Length: 288 bp

>T152992 NZ_CP066712:973998-974159 [Staphylococcus pseudintermedius]
ATGAAAGACGAACAAAAGCATTATGATAATGAAATGGTTGATAGTTTTGATGATGTTGTGGAGCTCGGTAAAGAAATGGA
ACAAATTTCTGAAGCGAACGATGAAGAGAAACTCAATCAAGCACATGATTCCAAAGTGCGTTCTGATAAAAATACAAAAT
AA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11397.92 Da
• Isoelectric Point: 9.3974

>AT152992 WP_148251187.1 NZ_CP050508:c4931948-4931646 [Raoultella terrigena]
MSTVKYKRDNPAGLSAQREDELRALAKKADGEIDYSDIPASGDEQWPDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLSEK

Nucleotide

• Length: 303 bp

>AT152992 NZ_CP066712:973378-973977 [Staphylococcus pseudintermedius]
ATGGCAATGAATTTTAAAGTATTTAAAGATGCAGAAACAGCAGCAACTTTCACAGCAGATATCTTAAGAAAACAGTTAAA
TAACAATCCGACATCGATTGTAGGTCTTCATTTAAATCAAGAAGAAGCACCTGTATTAGATGCATTAAAAAAAGATGTCG
ATTGTCATAGCGTTGATTTCAGTCAAATTCATGTATTGGATTATGACGCGCAACAATCATATTATCAAGCATTAGGTGTC
CCAGAAAAACAAATTCATCACGTACCAGAAGATGAAAAAGTGGAATCATTTATTAAACATCACGCAAAAACAAAAGATAA
TAAAGGTAAATTGACGTTACAAGTCGTGACAATCGATACAAAAGGTCAAATCGGTATACCTATGAATGACGCATTGTTAC
CAGCGCGTGAAATTATTGTTGTCGTGACAGGTGCAGTGAAAGCAGAACAAGTGAAGAAATTATACGAAGAAAACGGTAAT
ACGACATTTATTCCGTCAGCATTAAAATCACATCGTATGGTAACTGTTGTCCTTGATGAAGCAGCTGCACAAGGTTTACC
TGAAGATGTGCGCAATTACTTTACTTCATTGTACGCATAA