Detailed information of TA system TA152977

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 858549..859206
• Replicon: chromosome
• Accession: NZ_CP050508
• Organism: Raoultella terrigena strain JH01

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A4V6J1V0
• Locus tag: HCK03_RS04180
• Protein ID: WP_041147144.1
• Coordinates: 858796..859206 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HCK03_RS04175
• Protein ID: WP_041147145.1
• Coordinates: 858549..858815 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCK03_RS04150	853627..855060	-	1434	WP_041147149.1	6-phospho-beta-glucosidase	-
HCK03_RS04155	855181..855909	-	729	WP_045857150.1	MurR/RpiR family transcriptional regulator	-
HCK03_RS04160	855959..856270	+	312	WP_076948127.1	N(4)-acetylcytidine aminohydrolase	-
HCK03_RS04165	856430..857089	+	660	WP_041147747.1	hemolysin III family protein	-
HCK03_RS04170	857197..858180	-	984	WP_167691177.1	tRNA-modifying protein YgfZ	-
HCK03_RS04175	858549..858815	+	267	WP_041147145.1	FAD assembly factor SdhE	Antitoxin
HCK03_RS04180	858796..859206	+	411	WP_041147144.1	protein YgfX	Toxin
HCK03_RS04185	859255..859776	-	522	WP_041147143.1	flavodoxin FldB	-
HCK03_RS04190	859935..860831	+	897	WP_041147142.1	site-specific tyrosine recombinase XerD	-
HCK03_RS04195	860855..861568	+	714	WP_076948124.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HCK03_RS04200	861574..863307	+	1734	WP_041147140.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16133.03 Da
• Isoelectric Point: 11.3523

>T152977 WP_041147144.1 NZ_CP050508:858796-859206 [Raoultella terrigena]
VVLWQSDLRISWRSQWFSLLLHGIVAAIVLLMPWPLSYTPLWLLLLSLVVFDSVRSQRRIHACQGEIKLMTDSRLRWQKA
EWEIVGMPWVINSGMLLRLRHPQTRRSQHLWVAADSMDAKEWRDLRRLVLNKPAQD

Nucleotide

• Length: 411 bp

>T152977 NZ_CP066705:773269-773490 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10554.07 Da
• Isoelectric Point: 4.8432

>AT152977 WP_041147145.1 NZ_CP050508:858549-858815 [Raoultella terrigena]
MDITNKARIHWACRRGMRELDISIMPFFEYEYDTLSDEDKLLFIRLLENDDPDLFNWLMNHGEPSDAQLQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT152977 NZ_CP066705:772969-773256 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: A0A4V6J1V0