Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 152847..153373 Replicon plasmid pCM18-242-2
Accession NZ_CP050507
Organism Enterobacter hormaechei strain CM18-242-2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HCU35_RS22820 Protein ID WP_000323025.1
Coordinates 153086..153373 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HCU35_RS22815 Protein ID WP_000534858.1
Coordinates 152847..153086 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCU35_RS22800 149175..150413 + 1239 WP_000219087.1 IS110 family transposase -
HCU35_RS22805 150889..151461 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HCU35_RS22810 151661..152584 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
HCU35_RS22815 152847..153086 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HCU35_RS22820 153086..153373 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HCU35_RS22825 153445..153603 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
HCU35_RS22830 154212..154532 - 321 WP_000332796.1 hypothetical protein -
HCU35_RS22835 155064..155414 - 351 WP_000743059.1 hypothetical protein -
HCU35_RS22840 155474..156076 - 603 WP_012695474.1 hypothetical protein -
HCU35_RS22845 156172..157116 - 945 WP_000778029.1 DUF5417 domain-containing protein -
HCU35_RS22850 157627..157803 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / catA2 / tet(D) / sul1 / qacE / ere(A) / aac(6')-IIc / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrB2 / aadA2 / blaSHV-12 - 1..288061 288061


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T152976 WP_000323025.1 NZ_CP050507:153086-153373 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T152976 NZ_CP066703:788104-788457 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT152976 WP_000534858.1 NZ_CP050507:152847-153086 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT152976 NZ_CP066703:787933-788103 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References