Detailed information of TA system TA152975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93050..94673
• Replicon: plasmid pCM18-242-2
• Accession: NZ_CP050507
• Organism: Enterobacter hormaechei strain CM18-242-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: HCU35_RS22460
• Protein ID: WP_000088044.1
• Coordinates: 93348..94673 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: HCU35_RS22455
• Protein ID: WP_012477377.1
• Coordinates: 93050..93343 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCU35_RS22435	88849..89241	+	393	WP_000341066.1	hypothetical protein	-
HCU35_RS22440	89900..90556	+	657	WP_000462754.1	trypsin-like serine protease	-
HCU35_RS22445	90759..91256	+	498	WP_000340139.1	membrane protein	-
HCU35_RS22450	91261..92649	+	1389	WP_012695445.1	hypothetical protein	-
HCU35_RS22455	93050..93343	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
HCU35_RS22460	93348..94673	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
HCU35_RS22465	94734..94940	+	207	WP_000134171.1	hypothetical protein	-
HCU35_RS22470	95041..95451	+	411	WP_000985909.1	hypothetical protein	-
HCU35_RS22475	95464..95892	+	429	Protein_91	hypothetical protein	-
HCU35_RS22480	96170..97174	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
HCU35_RS22485	97265..97699	-	435	WP_000405672.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / catA2 / tet(D) / sul1 / qacE / ere(A) / aac(6')-IIc / mcr-9 / aph(6)-Id / aph(3'')-Ib / dfrA19 / qnrB2 / aadA2 / blaSHV-12	-	1..288061	288061
	-	flank	IS/Tn	-	-	96170..97174	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T152975 WP_000088044.1 NZ_CP050507:93348-94673 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T152975 NZ_CP066703:773318-773539 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT152975 WP_012477377.1 NZ_CP050507:93050-93343 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT152975 NZ_CP066703:773018-773305 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7