Detailed information of TA system TA152974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4606786..4607402
• Replicon: chromosome
• Accession: NZ_CP050506
• Organism: Enterobacter hormaechei strain CM18-242-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HCU35_RS21630
• Protein ID: WP_017382676.1
• Coordinates: 4606786..4607157 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HCU35_RS21635
• Protein ID: WP_015569912.1
• Coordinates: 4607160..4607402 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCU35_RS21615	4604286..4605188	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
HCU35_RS21620	4605185..4605820	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
HCU35_RS21625	4605817..4606746	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
HCU35_RS21630	4606786..4607157	-	372	WP_017382676.1	type II toxin-antitoxin system VapC family toxin	Toxin
HCU35_RS21635	4607160..4607402	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HCU35_RS21640	4607601..4608521	+	921	WP_017382675.1	alpha/beta hydrolase	-
HCU35_RS21645	4608530..4609471	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HCU35_RS21650	4609516..4609953	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HCU35_RS21655	4609950..4610831	-	882	WP_003861949.1	virulence factor BrkB family protein	-
HCU35_RS21660	4610825..4611424	-	600	WP_003861947.1	glucose-1-phosphatase	-
HCU35_RS21665	4611543..4612343	-	801	WP_017382673.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T152974 WP_017382676.1 NZ_CP050506:c4607157-4606786 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T152974 NZ_CP066702:788066-788419 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT152974 WP_015569912.1 NZ_CP050506:c4607402-4607160 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT152974 NZ_CP066702:787895-788065 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3