Detailed information of TA system TA152964

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1121367..1122150
• Replicon: chromosome
• Accession: NZ_CP050506
• Organism: Enterobacter hormaechei strain CM18-242-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A4R0G0V7
• Locus tag: HCU35_RS05285
• Protein ID: WP_004136885.1
• Coordinates: 1121367..1121741 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HCU35_RS05290
• Protein ID: WP_087528994.1
• Coordinates: 1121791..1122150 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCU35_RS05265	1116920..1118281	-	1362	WP_087528999.1	hypothetical protein	-
HCU35_RS05270	1118754..1119074	-	321	WP_047720740.1	hypothetical protein	-
HCU35_RS05275	1119839..1120828	+	990	WP_202768585.1	glycosyltransferase	-
HCU35_RS05280	1120967..1121287	+	321	WP_004136883.1	hypothetical protein	-
HCU35_RS05285	1121367..1121741	-	375	WP_004136885.1	TA system toxin CbtA family protein	Toxin
HCU35_RS05290	1121791..1122150	-	360	WP_087528994.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HCU35_RS05295	1122173..1122394	-	222	WP_064371656.1	DUF987 domain-containing protein	-
HCU35_RS05300	1122408..1122890	-	483	WP_202768587.1	DNA repair protein RadC	-
HCU35_RS05305	1123131..1123604	-	474	WP_202768589.1	antirestriction protein	-
HCU35_RS05310	1123683..1124504	-	822	WP_004136895.1	DUF945 domain-containing protein	-
HCU35_RS05315	1124611..1124823	-	213	WP_047720745.1	hypothetical protein	-
HCU35_RS05320	1124909..1125268	-	360	WP_004136901.1	hypothetical protein	-
HCU35_RS05325	1125323..1125787	-	465	WP_004136903.1	hypothetical protein	-
HCU35_RS05330	1126065..1126922	-	858	WP_025107340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1106535..1144511	37976

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-112)

Antitoxin

• Antitoxin CbeA (17-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13889.84 Da
• Isoelectric Point: 9.8173

>T152964 WP_004136885.1 NZ_CP050506:c1121741-1121367 [Enterobacter hormaechei]
MQIQSLSPQRAASSRLSSVESWRKLLEYLLKQHYGLMMNDTPFGDDSVIQKNIDAGISLCDAVNFIVEKYGLVRTDRHGF
SVTEQSPFIDSIDMLRARKATGLMTRKGYQTVTDITSGRSRGGK

Nucleotide

• Length: 375 bp

>T152964 NZ_CP066670:c12355-12005 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13334.46 Da
• Isoelectric Point: 7.7515

>AT152964 WP_087528994.1 NZ_CP050506:c1122150-1121791 [Enterobacter hormaechei]
MQKATRVINHNIAEPWRGLRRNITPCFGARLVQEGNHLHYFADRASIAGTFNEADLRYLDQAFPVLMKQMELMLASSELT
PHIQRCITLHAKGLICEADTLGSCGYLFIVIYPTSAISA

Nucleotide

• Length: 360 bp

>AT152964 NZ_CP066670:c12612-12349 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A4R0G0V7