Detailed information of TA system TA152957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2353753..2354419
• Replicon: chromosome
• Accession: NZ_CP050500
• Organism: Lacticaseibacillus paracasei strain NFFJ04

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HCJ88_RS11540
• Protein ID: WP_193137151.1
• Coordinates: 2353753..2354151 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K6RKI5
• Locus tag: HCJ88_RS11545
• Protein ID: WP_003567665.1
• Coordinates: 2354171..2354419 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCJ88_RS11535	2353063..2353584	-	522	WP_193137150.1	QueT transporter family protein	-
HCJ88_RS11540	2353753..2354151	-	399	WP_193137151.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HCJ88_RS11545	2354171..2354419	-	249	WP_003567665.1	antitoxin	Antitoxin
HCJ88_RS11550	2354487..2355623	-	1137	WP_016369534.1	alanine racemase	-
HCJ88_RS11555	2355610..2355984	-	375	WP_003567669.1	holo-ACP synthase	-
HCJ88_RS11560	2356153..2357661	-	1509	WP_003567670.1	DEAD/DEAH box helicase	-
HCJ88_RS11565	2357787..2357930	-	144	WP_003567673.1	hypothetical protein	-
HCJ88_RS11570	2357961..2359349	-	1389	WP_016369535.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14384.69 Da
• Isoelectric Point: 11.1283

>T152957 WP_193137151.1 NZ_CP050500:c2354151-2353753 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKNQQQRA

Nucleotide

• Length: 399 bp

>T152957 NZ_CP066659:191154-191420 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCACCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8938.08 Da
• Isoelectric Point: 4.4610

>AT152957 WP_003567665.1 NZ_CP050500:c2354419-2354171 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT152957 NZ_CP066659:190891-191154 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
AATATTGATTGGGAAGCTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2BNY2