Detailed information of TA system TA152955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4999444..5000069
• Replicon: chromosome
• Accession: NZ_CP050498
• Organism: Escherichia coli strain RM13322

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HCR07_RS25290
• Protein ID: WP_000911330.1
• Coordinates: 4999671..5000069 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HCR07_RS25285
• Protein ID: WP_000450524.1
• Coordinates: 4999444..4999671 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCR07_RS25260	4995246..4995716	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HCR07_RS25265	4995716..4996288	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HCR07_RS25270	4996434..4997312	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HCR07_RS25275	4997329..4998363	+	1035	WP_001368798.1	outer membrane protein assembly factor BamC	-
HCR07_RS25280	4998576..4999289	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HCR07_RS25285	4999444..4999671	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HCR07_RS25290	4999671..5000069	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HCR07_RS25295	5000216..5001079	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
HCR07_RS25300	5001094..5003109	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
HCR07_RS25305	5003183..5003881	+	699	WP_000679812.1	esterase	-
HCR07_RS25310	5003991..5004191	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T152955 WP_000911330.1 NZ_CP050498:4999671-5000069 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T152955 NZ_CP066653:516670-517011 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT152955 WP_000450524.1 NZ_CP050498:4999444-4999671 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT152955 NZ_CP066653:516440-516670 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33