Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2814681..2815276 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HCR07_RS14630 Protein ID WP_000239579.1
Coordinates 2814681..2815031 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HCR07_RS14635 Protein ID WP_001223208.1
Coordinates 2815025..2815276 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS14610 2810127..2811149 - 1023 WP_001298926.1 ABC transporter permease -
HCR07_RS14615 2811163..2812665 - 1503 WP_000205800.1 sugar ABC transporter ATP-binding protein -
HCR07_RS14620 2812805..2813761 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HCR07_RS14625 2814071..2814601 + 531 WP_000055072.1 inorganic diphosphatase -
HCR07_RS14630 2814681..2815031 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HCR07_RS14635 2815025..2815276 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HCR07_RS14640 2815489..2815830 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HCR07_RS14645 2815833..2819612 - 3780 WP_136766006.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T152945 WP_000239579.1 NZ_CP050498:c2815031-2814681 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T152945 NZ_CP066637:215590-215856 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT152945 WP_001223208.1 NZ_CP050498:c2815276-2815025 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT152945 NZ_CP066637:215327-215590 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References