152942

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2177300..2178137 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name itaT Uniprot ID A0A0F5GET7
Locus tag HCR07_RS11710 Protein ID WP_000227783.1
Coordinates 2177595..2178137 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HCR07_RS11705 Protein ID WP_001297137.1
Coordinates 2177300..2177611 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS11680 2172320..2173267 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HCR07_RS11685 2173289..2175280 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HCR07_RS11690 2175270..2175884 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HCR07_RS11695 2175884..2176213 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HCR07_RS11700 2176225..2177115 + 891 WP_000971336.1 heme o synthase -
HCR07_RS11705 2177300..2177611 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HCR07_RS11710 2177595..2178137 + 543 WP_000227783.1 GNAT family N-acetyltransferase Toxin
HCR07_RS11715 2178193..2179128 - 936 WP_001297127.1 sel1 repeat family protein -
HCR07_RS11720 2179536..2180900 + 1365 WP_001000978.1 MFS transporter -
HCR07_RS11725 2181028..2181519 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HCR07_RS11730 2181687..2182598 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19776.96 Da        Isoelectric Point: 8.0330

>T152942 WP_000227783.1 NZ_CP050498:2177595-2178137 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T152942 NZ_CP066629:218876-219142 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT152942 WP_001297137.1 NZ_CP050498:2177300-2177611 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT152942 NZ_CP066629:218613-218876 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F5GET7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References