Detailed information of TA system TA152939

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1188109..1188904
• Replicon: chromosome
• Accession: NZ_CP050498
• Organism: Escherichia coli strain RM13322

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: HCR07_RS06480
• Protein ID: WP_000854914.1
• Coordinates: 1188109..1188483 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LM11
• Locus tag: HCR07_RS06485
• Protein ID: WP_001280953.1
• Coordinates: 1188530..1188904 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCR07_RS06440	1183113..1183604	-	492	WP_001297187.1	DUF1097 domain-containing protein	-
HCR07_RS06445	1183706..1184260	-	555	WP_001001902.1	molecular chaperone YcdY	-
HCR07_RS06450	1184284..1185021	-	738	WP_000283664.1	zinc-binding phosphatase	-
HCR07_RS06455	1185076..1186014	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HCR07_RS06465	1186485..1187327	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
HCR07_RS06470	1187412..1187609	-	198	WP_000839282.1	DUF957 domain-containing protein	-
HCR07_RS06475	1187621..1188112	-	492	WP_000976857.1	hypothetical protein	-
HCR07_RS06480	1188109..1188483	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
HCR07_RS06485	1188530..1188904	-	375	WP_001280953.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HCR07_RS06490	1189067..1189288	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HCR07_RS06495	1189351..1189827	-	477	WP_001186738.1	RadC family protein	-
HCR07_RS06500	1189843..1190328	-	486	WP_000214398.1	antirestriction protein	-
HCR07_RS06505	1190419..1191237	-	819	WP_001234682.1	DUF945 domain-containing protein	-
HCR07_RS06510	1191577..1192647	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
HCR07_RS06515	1192644..1193549	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgC / csgA / csgB / csgD / csgE / csgF / csgG	1178608..1190328	11720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T152939 WP_000854914.1 NZ_CP050498:c1188483-1188109 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T152939 NZ_CP066622:514376-514717 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13734.56 Da
• Isoelectric Point: 6.6249

>AT152939 WP_001280953.1 NZ_CP050498:c1188904-1188530 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Nucleotide

• Length: 375 bp

>AT152939 NZ_CP066622:514146-514376 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9LM11