Detailed information of TA system TA152925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 472822..473438
• Replicon: chromosome
• Accession: NZ_CP050493
• Organism: Lentilactobacillus parabuchneri strain NSMJ16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HCC74_RS02290
• Protein ID: WP_057910635.1
• Coordinates: 472822..473004 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HCC74_RS02295
• Protein ID: WP_057910636.1
• Coordinates: 473040..473438 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCC74_RS02275	468086..468844	-	759	WP_057910632.1	hypothetical protein	-
HCC74_RS02280	468966..471365	-	2400	WP_057910633.1	phosphoketolase family protein	-
HCC74_RS02285	471795..472649	-	855	WP_057910634.1	metallophosphoesterase	-
HCC74_RS02290	472822..473004	+	183	WP_057910635.1	type II toxin-antitoxin system HicA family toxin	Toxin
HCC74_RS02295	473040..473438	+	399	WP_057910636.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HCC74_RS02300	473561..474640	-	1080	WP_057910637.1	aspartate-semialdehyde dehydrogenase	-
HCC74_RS02305	474661..475848	-	1188	WP_057910638.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
HCC74_RS02310	475870..476643	-	774	WP_057910639.1	4-hydroxy-tetrahydrodipicolinate reductase	-
HCC74_RS02315	476700..477614	-	915	WP_057910640.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-113)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7053.28 Da
• Isoelectric Point: 10.8513

>T152925 WP_057910635.1 NZ_CP050493:472822-473004 [Lentilactobacillus parabuchneri]
MDSRTIIQMLKHDGWYKVRIHGDHWHFKHPQKLGIVTVPHPRKDIPKGTYNSILKQAGLK

Nucleotide

• Length: 183 bp

>T152925 NZ_CP066594:33968-34225 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.02 Da
• Isoelectric Point: 6.2197

>AT152925 WP_057910636.1 NZ_CP050493:473040-473438 [Lentilactobacillus parabuchneri]
MKYLYYALFKKQSDDKIEVTFPDLAPHAATYGTTMSDALHMAHDALEGYLLVVEDLHEKLPKASSPESLTHDTSQLIIPI
EVDTTLAREREDNVSIKKTLTIPKYLNDLGKQKSINFSATLTDALKHKLNIL

Nucleotide

• Length: 399 bp

>AT152925 NZ_CP066594:33706-33975 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA