Detailed information of TA system TA152924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 45516..46087
• Replicon: plasmid pEGM183
• Accession: NZ_CP050492
• Organism: Enterococcus faecalis strain EGM183

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EGM183_RS13055
• Protein ID: WP_033593835.1
• Coordinates: 45746..46087 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: EGM183_RS13050
• Protein ID: WP_002362431.1
• Coordinates: 45516..45746 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM183_RS13020	42604..42921	+	318	WP_002394802.1	heavy metal-binding domain-containing protein	-
EGM183_RS13025	42957..43625	+	669	WP_002397511.1	CPBP family intramembrane metalloprotease	-
EGM183_RS13030	43742..43996	+	255	WP_002394800.1	hypothetical protein	-
EGM183_RS13035	44156..44389	-	234	WP_002397510.1	hypothetical protein	-
EGM183_RS13040	44391..44675	-	285	WP_002394798.1	hypothetical protein	-
EGM183_RS13045	44692..45312	-	621	WP_013438829.1	recombinase family protein	-
EGM183_RS13050	45516..45746	+	231	WP_002362431.1	hypothetical protein	Antitoxin
EGM183_RS13055	45746..46087	+	342	WP_033593835.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EGM183_RS13060	46199..47137	+	939	WP_033593837.1	hypothetical protein	-
EGM183_RS13065	47407..48009	+	603	WP_033593839.1	Fic family protein	-
EGM183_RS13070	48264..49025	-	762	WP_033593842.1	hypothetical protein	-
EGM183_RS13075	49217..49450	+	234	WP_050443865.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / tet(L)	-	1..73653	73653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13216.39 Da
• Isoelectric Point: 8.8676

>T152924 WP_033593835.1 NZ_CP050492:45746-46087 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYDFNRKTMFAVICPITSTIRNMPTRYTLPDEIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T152924 NZ_CP066593:c880-758 [Enterococcus faecium]
ATGAAAGGAGTAAGCCTTTTGTCCGCATATGAGACAATTCAAACCATTCTTGGTTTTGGTATGTTTACCATTGCTTTGAT
TAGTTTGATTGTAAATATGCTTAAAAATGACAAAAAGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT152924 WP_002362431.1 NZ_CP050492:45516-45746 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT152924 NZ_CP066593:675-776 [Enterococcus faecium]
CCCTCATTAACAGCACCGTTTAAAGAACGGCGGCTCAGATTAATTATTTATTGAGAAATAACCGATCCTCGCTAAAGTGA
CGGTTATTTCTTTTTGTCATTT

Structures

Antitoxin

• AlphaFold DB: R3KHK9