Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 5548..6685 Replicon plasmid pEGM181-1
Accession NZ_CP050484
Organism Enterococcus gallinarum strain EGM181

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag EGM181_RS00040 Protein ID WP_001284311.1
Coordinates 5822..6685 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag EGM181_RS00035 Protein ID WP_000301765.1
Coordinates 5548..5820 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM181_RS00010 1280..1450 + 171 WP_000713594.1 hypothetical protein -
EGM181_RS00015 1464..2081 + 618 WP_001062589.1 recombinase family protein -
EGM181_RS00020 2081..4225 + 2145 WP_000108741.1 type IA DNA topoisomerase -
EGM181_RS00025 4328..5224 + 897 WP_001835294.1 ParA family protein -
EGM181_RS00030 5322..5531 + 210 WP_000527318.1 peptide-binding protein -
EGM181_RS00035 5548..5820 + 273 WP_000301765.1 antitoxin Antitoxin
EGM181_RS00040 5822..6685 + 864 WP_001284311.1 toxin zeta Toxin
EGM181_RS00045 7023..7703 + 681 WP_000191454.1 IS6-like element ISEnfa1 family transposase -
EGM181_RS00050 7981..8313 + 333 WP_000713503.1 hypothetical protein -
EGM181_RS00055 8332..8715 + 384 WP_101143884.1 glycosyltransferase -
EGM181_RS00060 8732..9361 + 630 WP_101143883.1 hypothetical protein -
EGM181_RS00065 9372..11333 + 1962 WP_002301631.1 TrsE protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..24463 24463
- flank IS/Tn - - 7017..7703 686


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T152909 WP_001284311.1 NZ_CP050484:5822-6685 [Enterococcus gallinarum]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T152909 NZ_CP066568:515915-516256 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT152909 WP_000301765.1 NZ_CP050484:5548-5820 [Enterococcus gallinarum]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT152909 NZ_CP066568:515685-515915 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References