Detailed information of TA system TA152908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 20325..21672
• Replicon: plasmid unnamed1
• Accession: NZ_CP050477
• Organism: Vibrio campbellii strain LJC012

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: HB763_RS25205
• Protein ID: WP_255987063.1
• Coordinates: 20325..21266 (-)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: HB763_RS25210
• Protein ID: WP_255987064.1
• Coordinates: 21268..21672 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB763_RS25190 (HB763_25160)	16202..17380	-	1179	WP_255987079.1	AAA family ATPase	-
HB763_RS25195 (HB763_25165)	17390..18658	-	1269	WP_255987060.1	restriction endonuclease subunit S	-
HB763_RS25200 (HB763_25170)	18655..20211	-	1557	WP_255987061.1	type I restriction-modification system subunit M	-
HB763_RS25205 (HB763_25175)	20325..21266	-	942	WP_255987063.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HB763_RS25210 (HB763_25180)	21268..21672	-	405	WP_255987064.1	DUF6088 family protein	Antitoxin
HB763_RS25215 (HB763_25185)	21861..22453	-	593	Protein_18	tyrosine-type recombinase/integrase	-
HB763_RS25220 (HB763_25190)	22665..22919	-	255	WP_228176178.1	hypothetical protein	-
HB763_RS25225	22869..22994	+	126	WP_255987080.1	hypothetical protein	-
HB763_RS25230 (HB763_25195)	23000..23638	-	639	WP_228176122.1	recombinase family protein	-
HB763_RS25235 (HB763_25200)	23722..24212	-	491	Protein_22	recombinase family protein	-
HB763_RS25240 (HB763_25205)	24283..25329	-	1047	WP_228176130.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34734	34734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-300)

Antitoxin

• Protein of unknown function DUF6088 (7-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35867.34 Da
• Isoelectric Point: 5.6657

>T152908 WP_255987063.1 NZ_CP050477:c21266-20325 [Vibrio campbellii]
MNTLKQQFLEVSDALELGNPAIIEKDYWVVALLAELAKVCPVHHQMVFSGGTALAKSNVKILRMSEDVDIKLIPNPAFDG
LSRAKKKAARKACVNEIEQAIIHSERFTVETRSVRDEYRYVEFELKYPQQFSQAPCLRPIIKLELIETIPLLDVESRSIQ
SLVAELYQQPQEVSAFDCVSIHATLVEKIISMLRRTMSVKRNTERSDDVALVRHIYDVHCITKLSMPELNEIDGLFKAVL
EEDVVRFGNQHAEFVSNPKQELQLGLQELKENPVFRQRFQDFVTPMVFNTEPHDFDTCFTSFRNVAESLITKL

Nucleotide

• Length: 942 bp

>T152908 NZ_CP066568:216888-217154 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15167.56 Da
• Isoelectric Point: 10.6258

>AT152908 WP_255987064.1 NZ_CP050477:c21672-21268 [Vibrio campbellii]
MTAVDRICQKIKRSRRYVFERKDFDGFASYDQIGRALRQLVQQGELMKLGYGLYTKARINSLTGKPMPTNPGGTDALMRE
ILKMKGVDFEMDSLSLQSLSGTSTQIPSSIQYSWNPKQFNRKLVVGSRVLNLPS

Nucleotide

• Length: 405 bp

>AT152908 NZ_CP066568:216625-216888 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA