Detailed information of TA system TA152905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 83679..84265
• Replicon: plasmid unnamed2
• Accession: NZ_CP050473
• Organism: Vibrio campbellii strain LJC013

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HB762_RS28200
• Protein ID: WP_110097046.1
• Coordinates: 83936..84265 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HB762_RS28195
• Protein ID: WP_103412263.1
• Coordinates: 83679..83936 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB762_RS28165 (HB762_28015)	79437..79634	-	198	WP_255905341.1	hypothetical protein	-
HB762_RS28170 (HB762_28020)	79637..80560	-	924	WP_255905343.1	chemotaxis protein	-
HB762_RS28175 (HB762_28025)	80557..81297	-	741	WP_255905345.1	hypothetical protein	-
HB762_RS28180 (HB762_28030)	81297..82475	-	1179	WP_255905347.1	hypothetical protein	-
HB762_RS28185 (HB762_28035)	82472..82786	-	315	WP_012219870.1	hypothetical protein	-
HB762_RS28190 (HB762_28040)	82783..83409	-	627	WP_255905349.1	hypothetical protein	-
HB762_RS28195 (HB762_28045)	83679..83936	+	258	WP_103412263.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HB762_RS28200 (HB762_28050)	83936..84265	+	330	WP_110097046.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HB762_RS28205 (HB762_28055)	84282..84451	+	170	Protein_110	replicative DNA helicase	-
HB762_RS28210 (HB762_28060)	84597..84944	-	348	WP_255905351.1	hypothetical protein	-
HB762_RS28215 (HB762_28065)	85023..85754	-	732	WP_255905353.1	site-specific DNA-methyltransferase	-
HB762_RS28220 (HB762_28070)	86060..86347	-	288	WP_255905355.1	hypothetical protein	-
HB762_RS28225 (HB762_28075)	86350..86670	-	321	WP_255905357.1	hypothetical protein	-
HB762_RS28230 (HB762_28080)	86675..87088	-	414	WP_164368846.1	hypothetical protein	-
HB762_RS28235 (HB762_28085)	87187..87801	-	615	WP_255905359.1	tyrosine-type recombinase/integrase	-
HB762_RS28240 (HB762_28090)	88083..88355	+	273	WP_255905361.1	hypothetical protein	-
HB762_RS28245 (HB762_28095)	88321..88704	-	384	WP_255905363.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97344	97344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-99)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12116.17 Da
• Isoelectric Point: 9.6897

>T152905 WP_110097046.1 NZ_CP050473:83936-84265 [Vibrio campbellii]
MYVPDKGDIVSLDFDPSAGKEIMKRRPAFVISRKMFNEHTGFAVVAPITSTVRGMKLEVVLPEELSTQGAILIHQVKSLD
FSNRQVKFIEKAPQAITDKATELTKVIIS

Nucleotide

• Length: 330 bp

>T152905 NZ_CP066567:436798-437034 [Histophilus somni]
TTGAATCTCTATTTACAAAAACAGGTTTCACAAGATATCAAAAGGCGTATTGCTCCTTGCTTTACTGTCATTGATGAAAA
TAAACGTATTTTAGGCTACTATACTTTAGCCTCAACAAGCATTCCTCTTGTCTCTCTCCCTGAGAACCTTAAGAAAAAAT
TACCCCGTTATCCTAGTGTTCCGGCGGTTTTACTTGGACGCTTAGCCGTTGACAAGCAAGTCTCAATATTCATTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9270.77 Da
• Isoelectric Point: 4.5822

>AT152905 WP_103412263.1 NZ_CP050473:83679-83936 [Vibrio campbellii]
MTAVVIRQSGGANIVSIPKTIVKMLSLHAGSKLDLSVQDNRIVLTPVEEELTLEALLAESPKECFAITDEDREWINAKPV
GKEVI

Nucleotide

• Length: 258 bp

>AT152905 NZ_CP066567:437172-437435 [Histophilus somni]
ATGCGTACCGCTGCAATTAACCTACGAGTCCAGCCAAAGCAACGTGACTTAATTGATTATGCCGCTTCATTACTCGGAAA
AACACGTTCTGATTTTATGCTTGATGTTGCTTGCACTTATGCACAAAACGTGATTTTAGACAGAACAGTTTTTCAGCTTG
ATGAAGGAAAATTCAATCATTTTATTGAAGTACTTGAACAGCCTAATTTACCTAATGCCGGGTTGGAAAAATTAATGGCA
ACTCATTCACCTTGGGAATCATAA