Detailed information of TA system TA152904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 6910..7496
• Replicon: plasmid unnamed2
• Accession: NZ_CP050473
• Organism: Vibrio campbellii strain LJC013

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HB762_RS27725
• Protein ID: WP_110097046.1
• Coordinates: 7167..7496 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HB762_RS27720
• Protein ID: WP_103412263.1
• Coordinates: 6910..7167 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB762_RS27685 (HB762_27540)	2643..2867	-	225	WP_255905396.1	hypothetical protein	-
HB762_RS27690	2870..3046	-	177	WP_255905398.1	hypothetical protein	-
HB762_RS27695 (HB762_27545)	3027..3791	-	765	WP_255905400.1	Atg14 domain-containing protein	-
HB762_RS27700 (HB762_27550)	3788..4528	-	741	WP_255905345.1	hypothetical protein	-
HB762_RS27705 (HB762_27555)	4528..5706	-	1179	WP_255905347.1	hypothetical protein	-
HB762_RS27710 (HB762_27560)	5703..6017	-	315	WP_012219870.1	hypothetical protein	-
HB762_RS27715 (HB762_27565)	6014..6640	-	627	WP_255905349.1	hypothetical protein	-
HB762_RS27720 (HB762_27570)	6910..7167	+	258	WP_103412263.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HB762_RS27725 (HB762_27575)	7167..7496	+	330	WP_110097046.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HB762_RS27730 (HB762_27580)	7513..7682	+	170	Protein_15	replicative DNA helicase	-
HB762_RS27735 (HB762_27585)	7828..8175	-	348	WP_255905351.1	hypothetical protein	-
HB762_RS27740 (HB762_27590)	8254..8985	-	732	WP_255905353.1	site-specific DNA-methyltransferase	-
HB762_RS27745 (HB762_27595)	9291..9578	-	288	WP_255905355.1	hypothetical protein	-
HB762_RS27750 (HB762_27600)	9581..9901	-	321	WP_255905357.1	hypothetical protein	-
HB762_RS27755 (HB762_27605)	9906..10319	-	414	WP_164368846.1	hypothetical protein	-
HB762_RS27760 (HB762_27610)	10418..11557	-	1140	WP_255905402.1	tyrosine-type recombinase/integrase	-
HB762_RS27765 (HB762_27615)	11554..11937	-	384	WP_255905363.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97344	97344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-99)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12116.17 Da
• Isoelectric Point: 9.6897

>T152904 WP_110097046.1 NZ_CP050473:7167-7496 [Vibrio campbellii]
MYVPDKGDIVSLDFDPSAGKEIMKRRPAFVISRKMFNEHTGFAVVAPITSTVRGMKLEVVLPEELSTQGAILIHQVKSLD
FSNRQVKFIEKAPQAITDKATELTKVIIS

Nucleotide

• Length: 330 bp

>T152904 NZ_CP066566:1578389-1578649 [Histophilus somni]
ATGTACACTTTACGGTATAGTGATAAGGCGTTGAAGCAATTATGCAAGCTTGATAAAGGCACACAAAAGTTGATTTTGAC
TTGGATAGCAAATAACTTAGAGAACTGCGAAAATCCAAGAATAAAAGGCAAAGGACTTACTAGGAATCGAAGTGGAGAAT
GGCGGTATAGAGTAGGCGACTATCGGATTATTTGTGATATACGAGATAGTGAATTGATAATACTTGCCTTGTCTATTGGA
CACAGGAAAAATATTTATTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9270.77 Da
• Isoelectric Point: 4.5822

>AT152904 WP_103412263.1 NZ_CP050473:6910-7167 [Vibrio campbellii]
MTAVVIRQSGGANIVSIPKTIVKMLSLHAGSKLDLSVQDNRIVLTPVEEELTLEALLAESPKECFAITDEDREWINAKPV
GKEVI

Nucleotide

• Length: 258 bp

>AT152904 NZ_CP066566:1578168-1578389 [Histophilus somni]
ATGGCTATTTCAGTAAGACTAAACGCTCAAGACGAGCAATTATTTAAGTCCTATGCAAAAACAAATAATATTACGCTATC
CGAACTGTTCAGGAATGCCGTTTTTGAAAAAATCGAAGATGAATATGACCTTGCAGTATATCGTGAGTCTTTGGAAGACT
ACAACAAAACCCCAATGAGTTATTCTCACGCAGAGGTATGCAAGATGTTAGGAATTGAGTGA