Detailed information of TA system TA152901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1178270..1179187
• Replicon: chromosome
• Accession: NZ_CP050462
• Organism: Bacillus velezensis strain Htq6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HCC49_RS06265
• Protein ID: WP_057079950.1
• Coordinates: 1178441..1179187 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HCC49_RS06260
• Protein ID: WP_003154807.1
• Coordinates: 1178270..1178440 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCC49_RS06225	1173503..1175125	+	1623	WP_166706517.1	pyocin knob domain-containing protein	-
HCC49_RS06230	1175138..1175509	+	372	WP_070081681.1	hypothetical protein	-
HCC49_RS06235	1175514..1175711	+	198	WP_071391956.1	XkdX family protein	-
HCC49_RS06240	1175768..1176529	+	762	WP_094247020.1	phage portal protein	-
HCC49_RS06245	1176581..1176844	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HCC49_RS06250	1176858..1177121	+	264	WP_003154813.1	phage holin	-
HCC49_RS06255	1177135..1178013	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
HCC49_RS06260	1178270..1178440	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HCC49_RS06265	1178441..1179187	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HCC49_RS06270	1179292..1180290	-	999	WP_003154805.1	inorganic phosphate transporter	-
HCC49_RS06275	1180303..1180920	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HCC49_RS06280	1181206..1182522	-	1317	WP_003154801.1	amino acid permease	-
HCC49_RS06285	1182845..1183795	+	951	WP_039251754.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T152901 WP_057079950.1 NZ_CP050462:c1179187-1178441 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T152901 NZ_CP066565:c1724384-1723917 [Histophilus somni]
ATGTATTCAGCACCTGAGCTATTAACACATCATCACCATTTAGGTGCATTTAATTGTGGTGAAGAGTCATTAAATATTTG
GCTTAAACGTCATGCACTTAGAAACCGGCAAAACCGAGCAAGTAGAACATTTGTGATCTGCAATGAAAATGTCGTTGTTG
GCTTCTATGCACTAGCGGCAGGTTCAGTGACGCATCGGTTTGTATCCGGAGCATTAAGACGTAATATGCCTGAGCCTATT
CCTGTTGTTGTTTTAGGAAGATTAGCTATAGATATAAAACATCAAGGAAAAAAATTAGGTGCTGCATTACTTAAAGATGT
TGTACTACGAGCAAGAGCTGTGTCAGAACAAGTTGGAGTAAGAGCTTTGCTCGTACACGCTTTAAATGATAAAGCAAAAC
AATTCTATCTCAATTATGGTTTTCAACCTTCACCAATTGATGATATGTTATTGATGTTGAAGTTATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT152901 WP_003154807.1 NZ_CP050462:c1178440-1178270 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT152901 NZ_CP066565:c1724649-1724386 [Histophilus somni]
ATGCGTACCGCTGCAATTAACCTACGAGTCCAGCCAAAGCAACGTGACTTAATTGATTATGCCGCTTCATTACTCGGAAA
AACACGTTCTGATTTTATGCTTGATGTTGCTTGCACTTATGCACAAAACGTGATTTTAGACAGAACAGTTTTTCAGCTTG
ATGAAGGAAAATTCAATCATTTTATTGAAGTACTTGAACAGCCTAATTTACCTAATGCCGGGTTGGAAAAATTAATGGCA
ACTCATTCACCTTGGGAATCATAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14