Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 444538..445175 Replicon chromosome
Accession NZ_CP050462
Organism Bacillus velezensis strain Htq6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HCC49_RS02330 Protein ID WP_003156187.1
Coordinates 444825..445175 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HCC49_RS02325 Protein ID WP_003156188.1
Coordinates 444538..444819 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCC49_RS02305 440904..441503 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
HCC49_RS02310 441596..441961 + 366 WP_070081400.1 holo-ACP synthase -
HCC49_RS02315 442126..443133 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
HCC49_RS02320 443249..444418 + 1170 WP_146276152.1 alanine racemase -
HCC49_RS02325 444538..444819 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HCC49_RS02330 444825..445175 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HCC49_RS02335 445293..446114 + 822 WP_003156182.1 STAS domain-containing protein -
HCC49_RS02340 446119..446484 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HCC49_RS02345 446487..446888 + 402 WP_003156178.1 anti-sigma regulatory factor -
HCC49_RS02350 446900..447907 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
HCC49_RS02355 447971..448300 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HCC49_RS02360 448297..448779 + 483 WP_003156173.1 anti-sigma B factor RsbW -
HCC49_RS02365 448745..449533 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
HCC49_RS02370 449533..450135 + 603 WP_146276154.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T152900 WP_003156187.1 NZ_CP050462:444825-445175 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T152900 NZ_CP066565:c1359539-1359234 [Histophilus somni]
ATGTCTGGTAAACAATTAAAGGTCAGTTACTCTAAACAATTTGCTAGAGATTTGACTGATTTGGCAAAACGTTACCCAAA
TGTGCTAGTAGGAACGAAATATATTACAGCAATGCACTGCTTGTTAAACCGCTTGCCATTGCCTGAAAGCTATCAAGATC
ACACATTAGTTGGAGAATGGAAAGGTTATCGAGATTGCCATATTCAAGGGGATTTGGTGCTGATTTACCAATACGTTATA
CACGATGAATTTGATGCGTTGAAATTTTCTCGTTTAAATACCTACTCGCAAACAGCTTCAAAATAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT152900 WP_003156188.1 NZ_CP050462:444538-444819 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT152900 NZ_CP066565:c1359833-1359543 [Histophilus somni]
ATGGCACTTACAAATGCGTCTATCAGCTTTAGAACAGTTGAGCAAACTAAATCCGAAGCCTATCAGGTCATTGAACAATA
TGGTCTTACACCATCACAAGTATTTAATATGTTTTTGGTTCAAATTGCCAAGACTCGCTCTATCCCTGTTGATTTGAATT
ATCTTCGCCCAAATAAAGAAACCTTAGCAGCTATGGACGAATTAGATAGCGGTAATGCAGAGAGCTTTTTTATTGAAGCT
GGAGAAAATTATTCTGTCGAAGAGTTTACAAAACGCATTCTTAACGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References