Detailed information of TA system TA152893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 490376..491013
• Replicon: chromosome
• Accession: NZ_CP050448
• Organism: Bacillus velezensis strain BIM B-1312D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HB674_RS02500
• Protein ID: WP_003156187.1
• Coordinates: 490663..491013 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HB674_RS02495
• Protein ID: WP_003156188.1
• Coordinates: 490376..490657 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB674_RS02475	486741..487340	-	600	WP_032872997.1	rhomboid family intramembrane serine protease	-
HB674_RS02480	487433..487798	+	366	WP_007609580.1	holo-ACP synthase	-
HB674_RS02485	487963..488970	+	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
HB674_RS02490	489087..490256	+	1170	WP_032873001.1	alanine racemase	-
HB674_RS02495	490376..490657	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HB674_RS02500	490663..491013	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HB674_RS02505	491131..491952	+	822	WP_003156182.1	STAS domain-containing protein	-
HB674_RS02510	491957..492322	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
HB674_RS02515	492325..492726	+	402	WP_003156178.1	anti-sigma regulatory factor	-
HB674_RS02520	492738..493745	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
HB674_RS02525	493809..494138	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
HB674_RS02530	494135..494617	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
HB674_RS02535	494583..495371	+	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
HB674_RS02540	495371..495973	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T152893 WP_003156187.1 NZ_CP050448:490663-491013 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T152893 NZ_CP066563:436536-437003 [Histophilus somni]
ATGTATTCAGCACCTGAGCTATTAACACATCATCACCATTTAGGTGCATTTAATTGTGGTGAAGAGTCATTAAATATTTG
GCTTAAACGTCATGCACTTAAAAACCGGCAAAACCGAGCAAGTAGAACATTTGTGATCTGCAATGAAAATGTCGTTGTTG
GCTTCTATGCACTAGCGGCAGGTTCAGTGACGCAGCGGTTTGTATCCGGAGCATTAAGACGTAATATGCCTGAGCCTATT
CCTGTTGTTGTTTTAGGAAGATTAGCTATAGATATAAAACATCAAGGAAAAAAATTAGGTGCTGCATTACTTAAAGATGT
TGTACTACGAGCAAGAGCTGTGTCAGAACAAGTTGGAGTAAGAGCTTTGCTCGTACACGCTTTAAATGATAAAGCAAAAC
AATTCTATCTCAATTATGGTTTTCAACCTTCGCCAATTGATGATATGTTATTGATGTTGAAGTTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT152893 WP_003156188.1 NZ_CP050448:490376-490657 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT152893 NZ_CP066563:436271-436534 [Histophilus somni]
ATGCGTACCGCTGCAATTAACCTACGAGTCCAGCCAAAGCAACGTGACTTAATTGATTATGCCGCTTCATTACTCGGAAA
AACACGTTCTGATTTTATGCTTGATGTTGCTTGCACTTATGCACAAAACGTGATTTTAGACAGAACAGTTTTTCAGCTTG
ATGAAGGAAAATTCAATCATTTTATTGAAGTACTTGAACAGCCTAATTTACCTAATGCCGGGTTGGAAAAATTAATGGCA
ACTCATTCACCTTGGGAATCATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5