Detailed information of TA system TA152859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1290301..1291218
• Replicon: chromosome
• Accession: NZ_CP050424
• Organism: Bacillus velezensis strain S4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BVELS4_RS06725
• Protein ID: WP_007407256.1
• Coordinates: 1290472..1291218 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BVELS4_RS06720
• Protein ID: WP_003154807.1
• Coordinates: 1290301..1290471 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVELS4_RS06685	1285514..1287103	+	1590	WP_167340618.1	hypothetical protein	-
BVELS4_RS06690	1287116..1287541	+	426	WP_051483351.1	hypothetical protein	-
BVELS4_RS06695	1287546..1287743	+	198	WP_007407259.1	XkdX family protein	-
BVELS4_RS06700	1287800..1288561	+	762	WP_007407258.1	hypothetical protein	-
BVELS4_RS06705	1288613..1288876	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BVELS4_RS06710	1288890..1289153	+	264	WP_003154813.1	phage holin	-
BVELS4_RS06715	1289167..1290045	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BVELS4_RS06720	1290301..1290471	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BVELS4_RS06725	1290472..1291218	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BVELS4_RS06730	1291323..1292321	-	999	WP_020955696.1	inorganic phosphate transporter	-
BVELS4_RS06735	1292334..1292951	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BVELS4_RS06740	1293237..1294553	-	1317	WP_007407254.1	amino acid permease	-
BVELS4_RS06745	1294876..1295826	+	951	WP_163113566.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T152859 WP_007407256.1 NZ_CP050424:c1291218-1290472 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T152859 NZ_CP066552:175246-176133 [Pectobacterium carotovorum]
ATGCTGAAGCAACAAATTCGCCAAATCGTCCAGTCAAACCCTGACTATGCCGCAGTGACACCCGTCATAGAAAAAGAGAT
CTTGCATCATGACATTATGGATGTGCTGATCAAACAGGGCGTCATGCAGCGGTTAACGTTTATTGGCGGTACCTCATTAC
GAATGTGTTACAACAGCAGCAGGCTATCAGAAGATCTTGATTTCAATGGCGGTCATGATTTCCAGCCCTCAGCGTTTGAG
GGGCTGGAGTCCGAAATTCAAACCTATATTCAGAACAAGTATGAAACCGAAGTTTGGGTAAACAAACCTTCTGGCGCGCA
GCAAGGGGATACCGTTTCTTGGAAAATTAGTATCGTAAAAGAAGCTAATCATCCTGATTTGCCACGGCAAAAAATGCATA
TTGACGTCTGTGCTATTCCGTCATTTGATATTGAAAAGCGCCCGCTAATCAATCATTACGACATCGTTGTACCGACTGAA
GGGATCTTGGTTCCGGTACAGTCGTTACAAGAAACATTGGCCGATAAGTTGATCGCGTTAGCGTATCGGGCAAGACGTAT
TAAACCTCGTGATATCTGGGATATTGTTTGGATTAAGCAGCGTGGTATTGATTTATCCAAAACACTGGTTGAAAAGAAGC
TCACCGCCAGACAGAAGCAAACAGAAGACTTTCGACAGGCGCTGTCTATTCAGTTAGCCAAGCTGATGTCGGAAGATGAA
GTCCGTGACGACTTCAATATGGAAATGAGCCGCTTTATTCCTAAGCAAATTAAAGAGAGAACCCTCGATAATCCTGAATA
TTGGGCTTATGTTCAGGGCGAGGTACATACCATCGCGTCTGAGTTACTGAATGACGGAACACCAAAAAATCCATTTGATA
TGGGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT152859 WP_003154807.1 NZ_CP050424:c1290471-1290301 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT152859 NZ_CP066552:174693-175217 [Pectobacterium carotovorum]
ATGGCTAAATCTGATGAGCTATTAAACGCGTTGGCTAAATCAGTGAACGCTGGCGGTGGCATTCACAGTGTAACAGAGCT
GGCTTTTATGCAGGGGGTGCCTTGTGACCCAGCCTTTAGAAAGTTCCTGGCAGATTGCGTAAAGAAGGGGTTGCTAAGAC
GAGTGGTGAAAGGCTTCTATGAAAGTGTTATTACACCACCTGAGCCTGAGACTGCCATTTATAAAATCATCAAAAAGTTG
CGCAGTGGCGTATTGAACTACATCAGTTTAGAAAGTCAGCTCAGTCATACCGGTGATATTTCACAAGTTGTGATGGGTAG
AGTCACGGTGGTGACTAAAGGTCGAAGCGGTTGTTTTGATACGCCTTATGGCGTGATCGAGCTTACCCATACTAAAAAGC
CAGTCGAGCAAATTGCACCTAATTTGTACTATGACCCCGACATCAAAATGTATCGCGCATACAAGGAACAAGCGATTGCA
GATCTAAAGCACTGCCAGCGCAATCTACATATGTTGGAGAGCTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14