Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 3997..4585 Replicon plasmid pPM193665_3
Accession NZ_CP050418
Organism Acinetobacter baumannii strain PM193665

Toxin (Protein)


Gene name brnT Uniprot ID A0A0J8TIH5
Locus tag HB367_RS20395 Protein ID WP_000438827.1
Coordinates 3997..4284 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9M5I3
Locus tag HB367_RS20400 Protein ID WP_001983304.1
Coordinates 4271..4585 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HB367_RS20375 1..924 + 924 WP_005174434.1 replication initiation protein RepM -
HB367_RS20380 1009..1551 + 543 WP_005174436.1 plasmid replication DNA-binding protein -
HB367_RS20385 1846..3225 + 1380 WP_031985417.1 ATP-binding protein -
HB367_RS20390 3218..3655 + 438 WP_031985418.1 hypothetical protein -
HB367_RS20395 3997..4284 + 288 WP_000438827.1 BrnT family toxin Toxin
HB367_RS20400 4271..4585 + 315 WP_001983304.1 BrnA antitoxin family protein Antitoxin
HB367_RS20405 4713..7124 + 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
HB367_RS20410 7429..7890 + 462 WP_000761346.1 DIP1984 family protein -
HB367_RS20415 8247..9412 - 1166 Protein_8 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..18783 18783


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11329.75 Da        Isoelectric Point: 5.6762

>T152853 WP_000438827.1 NZ_CP050418:3997-4284 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T152853 NZ_CP066546:c2410613-2410308 [Pseudomonas chlororaphis subsp. aureofaciens]
CTGGCAAGGCACGTTGAGTGGCTGCGCAGCGCCCTCAAGAACCTCGATGACGAAGCCGCCTACATTGCACAGGACAGTCC
TCAGGCCGCCGCGGAATTCGTCAAAGCCATCCTGAGCAGCATTCAACACCTGAGCCGTTTTCCTGCCAGTGGTCGCGAAG
GTCGCCTGCCCGGTACCCGGGAGTGGGTGGTCCCCAATCGTCCTTACCTGATCCCCTACCGTGTACGTCAGGGACACCTG
CAGATACTGCGGATTTTCCATACCCGGCGCTTGCCGCCTGCCGGTTGGCAAGACGACCGCCAATAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12208.84 Da        Isoelectric Point: 7.2026

>AT152853 WP_001983304.1 NZ_CP050418:4271-4585 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT152853 NZ_CP066546:c2410836-2410597 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGTCCGTCATGTCCTTGCGCCTGCCCGATGATGTTGCCAATTCCCTGGCTCACTTGGCCAAGGCGACAGGCCGCAGCAA
GTCATTCCTCGCTGTGGATGCACTGAGAGAATATCTGGCCCGCGAAGCCTGGCAAATCGACGAGATCCAGAAAGCCCTGA
CTGAAGCAGAGGCAGGCGACTTCGCCAGCGCCGAAGAGCTGGATAACGTGCTCACTAAATGGACTGGCAAGGCACGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J8TIH5

Antitoxin

Source ID Structure
AlphaFold DB N9M5I3

References