Detailed information of TA system TA152853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3997..4585
• Replicon: plasmid pPM193665_3
• Accession: NZ_CP050418
• Organism: Acinetobacter baumannii strain PM193665

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0J8TIH5
• Locus tag: HB367_RS20395
• Protein ID: WP_000438827.1
• Coordinates: 3997..4284 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: HB367_RS20400
• Protein ID: WP_001983304.1
• Coordinates: 4271..4585 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HB367_RS20375	1..924	+	924	WP_005174434.1	replication initiation protein RepM	-
HB367_RS20380	1009..1551	+	543	WP_005174436.1	plasmid replication DNA-binding protein	-
HB367_RS20385	1846..3225	+	1380	WP_031985417.1	ATP-binding protein	-
HB367_RS20390	3218..3655	+	438	WP_031985418.1	hypothetical protein	-
HB367_RS20395	3997..4284	+	288	WP_000438827.1	BrnT family toxin	Toxin
HB367_RS20400	4271..4585	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
HB367_RS20405	4713..7124	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
HB367_RS20410	7429..7890	+	462	WP_000761346.1	DIP1984 family protein	-
HB367_RS20415	8247..9412	-	1166	Protein_8	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18783	18783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11329.75 Da
• Isoelectric Point: 5.6762

>T152853 WP_000438827.1 NZ_CP050418:3997-4284 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T152853 NZ_CP066546:c2410613-2410308 [Pseudomonas chlororaphis subsp. aureofaciens]
CTGGCAAGGCACGTTGAGTGGCTGCGCAGCGCCCTCAAGAACCTCGATGACGAAGCCGCCTACATTGCACAGGACAGTCC
TCAGGCCGCCGCGGAATTCGTCAAAGCCATCCTGAGCAGCATTCAACACCTGAGCCGTTTTCCTGCCAGTGGTCGCGAAG
GTCGCCTGCCCGGTACCCGGGAGTGGGTGGTCCCCAATCGTCCTTACCTGATCCCCTACCGTGTACGTCAGGGACACCTG
CAGATACTGCGGATTTTCCATACCCGGCGCTTGCCGCCTGCCGGTTGGCAAGACGACCGCCAATAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT152853 WP_001983304.1 NZ_CP050418:4271-4585 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT152853 NZ_CP066546:c2410836-2410597 [Pseudomonas chlororaphis subsp. aureofaciens]
ATGTCCGTCATGTCCTTGCGCCTGCCCGATGATGTTGCCAATTCCCTGGCTCACTTGGCCAAGGCGACAGGCCGCAGCAA
GTCATTCCTCGCTGTGGATGCACTGAGAGAATATCTGGCCCGCGAAGCCTGGCAAATCGACGAGATCCAGAAAGCCCTGA
CTGAAGCAGAGGCAGGCGACTTCGCCAGCGCCGAAGAGCTGGATAACGTGCTCACTAAATGGACTGGCAAGGCACGTTGA

Structures

Toxin

• AlphaFold DB: A0A0J8TIH5

Antitoxin

• AlphaFold DB: N9M5I3