Detailed information of TA system TA152841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2433..3021
• Replicon: plasmid pVB2486_2
• Accession: NZ_CP050405
• Organism: Acinetobacter baumannii strain VB2486

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0J8TIH5
• Locus tag: HBN34_RS20580
• Protein ID: WP_000438827.1
• Coordinates: 2433..2720 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: HBN34_RS20585
• Protein ID: WP_001983304.1
• Coordinates: 2707..3021 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBN34_RS20565	1..924	+	924	WP_005174434.1	replication initiation protein RepM	-
HBN34_RS20570	1009..1551	+	543	WP_005174436.1	plasmid replication DNA-binding protein	-
HBN34_RS20575	1741..2160	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
HBN34_RS20580	2433..2720	+	288	WP_000438827.1	BrnT family toxin	Toxin
HBN34_RS20585	2707..3021	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
HBN34_RS20590	3149..5560	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
HBN34_RS20595	5865..6326	+	462	WP_000761346.1	DIP1984 family protein	-
HBN34_RS20600	6683..7848	-	1166	Protein_7	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14906	14906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11329.75 Da
• Isoelectric Point: 5.6762

>T152841 WP_000438827.1 NZ_CP050405:2433-2720 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T152841 NZ_CP066543:c3037514-3037412 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT152841 WP_001983304.1 NZ_CP050405:2707-3021 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT152841 NZ_CP066543:3037405-3037550 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0J8TIH5

Antitoxin

• AlphaFold DB: N9M5I3